通过比较琼脂扩散试验对几种白色念珠菌菌株进行氟康唑-两性霉素B拮抗作用的定量筛选。
Quantitative screening for fluconazole-amphotericin B antagonism in several Candida albicans strains by a comparative agar diffusion assay.
作者信息
Scheven M, Scheven C
机构信息
Medizinal-, Lebensmittel- und Veterinäruntersuchungsamt, Hygiene-Institut Gera, Germany.
出版信息
Mycoses. 1996 Mar-Apr;39(3-4):111-4. doi: 10.1111/j.1439-0507.1996.tb00111.x.
Antagonism between fluconazole (FCZ) and amphotericin B (AMB) was determined with an agar diffusion technique using series of agar plates containing no or 10 mgl-1 FCZ (comparative diffusion assay). Serial dilutions of AMB produced concentration-dependent inhibition zones that varied between the two agar plate series. This technique served as screening method to determine FCZ-AMB interactions in 18 Candida albicans strains. The critical concentrations of AMB were enhanced 1.33- to 7.0-fold by FCZ. The critical time, T0, was reduced by half by FCZ.
采用琼脂扩散技术，通过一系列不含氟康唑（FCZ）或含有10 mg/L氟康唑的琼脂平板（比较扩散试验），测定氟康唑（FCZ）与两性霉素B（AMB）之间的拮抗作用。两性霉素B的系列稀释液产生了浓度依赖性的抑菌圈，在两个琼脂平板系列中有所不同。该技术作为一种筛选方法，用于确定18株白色念珠菌中氟康唑与两性霉素B的相互作用。氟康唑使两性霉素B的临界浓度提高了1.33至7.0倍。氟康唑使临界时间T0缩短了一半。
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