Domnina L V, Ivanova O Iu, Vasil'ev Iu M
Tsitologiia. 1996;38(3):300-4.
The aim of this investigation was to study the effect of microtubule-specific drugs, taxol and colcemid, on the morphology of cytoskeletal systems and distribution of cell-associated extracellular matrix in dense cultures of fibroblasts. Immunomorphological examination of human 6-8 days old cultures revealed that microtubules, actin and vimentin filaments and filaments of extracellular matrix: fibronectin and tenascin-preferentially oriented in parallel with long axes of cell bodies. Depolymerization of microtubular system by colcemid and its disorganization by taxol lead to rapid and drastic changes in morphology of all cytoskeletal systems and in the organization of matrix network: fibronectin and tenascin filaments became disordered and, in particular, lost any orientation. These data show that microtubular system controls the morphological organization, and not only of two intracellular cytoskeletal systems, but also of extracellular matrix structures.
本研究的目的是研究微管特异性药物紫杉醇和秋水仙酰胺对成纤维细胞密集培养物中细胞骨架系统形态以及细胞相关细胞外基质分布的影响。对6 - 8日龄人类培养物的免疫形态学检查显示，微管、肌动蛋白和波形蛋白丝以及细胞外基质的丝：纤连蛋白和腱生蛋白 - 优先与细胞体的长轴平行排列。秋水仙酰胺使微管系统解聚，紫杉醇使其紊乱，导致所有细胞骨架系统的形态以及基质网络的组织发生快速而剧烈的变化：纤连蛋白和腱生蛋白丝变得无序，尤其是失去了任何方向。这些数据表明，微管系统不仅控制两种细胞内细胞骨架系统的形态组织，还控制细胞外基质结构的形态组织。
