Rocco M V, Buckalew V M, Moore L C, Shihabi Z K
Department of Internal Medicine/Nephrology, Baptist Hospital, Bowman Gray School of Medicine of Wake Forest University, Winston-Salem, NC 27157-1053, USA.
Am J Kidney Dis. 1996 Aug;28(2):173-7. doi: 10.1016/s0272-6386(96)90298-x.
High-performance liquid chromatography (HPLC) has been used as an alternative to the isotopic method to calculate glomerular filtration rate (GFR). With the HPLC method, serum iohexol or iothalamate levels are measured, and the plasma clearance rate of the compound is used as a surrogate for GFR. However, HPLC is a labor-intensive procedure, which limits its usefulness in the clinical setting. Capillary electrophoresis, a newer technique in which electrophoretic separations are performed in capillary tubes, is easier and faster than HPLC. We used capillary electrophoresis for the determination of serum iohexol levels and the calculation of GFR. Patients underwent a simultaneous 125I-iothalamate clearance test and a plasma iohexol clearance test to determine GFR. Mean GFR (+/-SD) was 70.9 +/- 29.9 mL/min (range, 14.5 to 131 mL/min) in 52 patients as determined by standard iothalamate clearance methods. For iohexol clearance, the correlation coefficient and standard error were 0.93 and 10.9 mL/min, respectively, using capillary electrophoresis compared with the iothalamate method. Capillary electrophoresis is a simple, rapid method that can be used to calculate GFR and provides results at least as accurate as those obtained by HPLC and x-ray fluorescence.
高效液相色谱法（HPLC）已被用作计算肾小球滤过率（GFR）的同位素方法的替代方法。使用HPLC方法时，需测量血清碘海醇或碘他拉酸盐水平，并将该化合物的血浆清除率用作GFR的替代指标。然而，HPLC是一项劳动强度大的操作，这限制了它在临床环境中的实用性。毛细管电泳是一种在毛细管中进行电泳分离的较新技术，比HPLC更简便、快速。我们使用毛细管电泳来测定血清碘海醇水平并计算GFR。患者同时接受125I-碘他拉酸盐清除试验和血浆碘海醇清除试验以确定GFR。通过标准碘他拉酸盐清除方法测定，52例患者的平均GFR（±标准差）为70.9±29.9 mL/分钟（范围为14.5至131 mL/分钟）。对于碘海醇清除率，与碘他拉酸盐方法相比，使用毛细管电泳时相关系数和标准误差分别为0.93和10.9 mL/分钟。毛细管电泳是一种简单、快速的方法，可用于计算GFR，其提供的结果至少与通过HPLC和X射线荧光法获得的结果一样准确。
