Kendall R W, Duncan C P, Smith J A, Ngui-Yen J H
Department of Orthopaedics, Vancouver Hospital, Canada.
Clin Orthop Relat Res. 1996 Aug(329):273-80. doi: 10.1097/00003086-199608000-00034.
Bacterial growth on the surface of antibiotic loaded acrylic cement was examined in an in vitro model. Tobramycin or vancomycin impregnated discs were incubated in broth containing either Staphylococcus epidermidis or Staphylococcus aureus organisms. At 24, 48, and 96 hours, the broth and the surface of the acrylic discs were examined for viable organisms. In the broth, only 6% (8 of 136) of samples contained viable organisms at 24 hours, and all samples were sterile by 96 hours. This is in contrast to the surface of the discs, which revealed the presence of viable organisms at all study time periods. Growth was noted in 99% and 30% of the Staphylococcus epidermidis and Staphylococcus aureus discs, respectively, at 24 hours. Viable organisms were found on both types of discs through 96 hours (20% of Staphylococcus epidermidis and 15% of Staphylococcus aureus). The surface of bone cement is therefore a suitable substrate for bacterial growth, even in the presence of antibiotics. In clinical practice, antibiotic loaded acrylic cement should therefore be used with caution and clear indications.
在体外模型中检测了载抗生素丙烯酸骨水泥表面的细菌生长情况。将妥布霉素或万古霉素浸渍的圆盘在含有表皮葡萄球菌或金黄色葡萄球菌的肉汤中孵育。在24、48和96小时时，检测肉汤和丙烯酸圆盘表面的活菌。在肉汤中，仅6%（136个样本中的8个）的样本在24小时时含有活菌，到96小时时所有样本均无菌。这与圆盘表面形成对比，圆盘表面在所有研究时间段均显示有活菌存在。在24小时时，分别在99%的表皮葡萄球菌圆盘和30%的金黄色葡萄球菌圆盘中观察到生长。在两种类型的圆盘上均发现活菌持续存在至96小时（表皮葡萄球菌为20%，金黄色葡萄球菌为15%）。因此，即使存在抗生素，骨水泥表面仍是细菌生长的合适基质。在临床实践中，因此应谨慎使用载抗生素丙烯酸骨水泥并有明确指征。
