Uherova P, Connick E, MaWhinney S, Schlichtemeier R, Schooley R T, Kuritzkes D R
Division of Infectious Diseases, University of Colorado Health Sciences Center, Denver 80262, USA.
J Infect Dis. 1996 Sep;174(3):483-9. doi: 10.1093/infdis/174.3.483.
The relationship between CD4 lymphocyte count and the in vitro effect of interleukin (IL)-12 on lymphocyte proliferative responses to Candida, tetanus toxoid, and streptokinase antigens was studied in peripheral blood mononuclear cells (PBMC from 30 human immunodeficiency virus (HIV)-infected persons and 10 seronegative controls. IL-12 significantly increased proliferative responses to microbial recall antigens of PBMC from HIV-infected persons with >200 CD4 lymphocytes/mm3 but had little effect on PBMC from patients with more advanced disease. The greatest increase was seen in patients with 200-500 CD4 cells/mm3. Results of limiting dilution analysis suggested that the increase in antigen-specific lymphocyte proliferation in the presence of IL-12 was due to an increase in the number of responding cells rather than an increase in the extent of proliferation of a fixed number of responder cells.
在30例人类免疫缺陷病毒（HIV）感染者及10例血清学阴性对照者的外周血单核细胞（PBMC）中，研究了CD4淋巴细胞计数与白细胞介素（IL）-12对淋巴细胞针对念珠菌、破伤风类毒素和链激酶抗原增殖反应的体外效应之间的关系。IL-12显著增强了CD4淋巴细胞计数>200/mm³的HIV感染者PBMC对微生物回忆抗原的增殖反应，但对病情更严重患者的PBMC影响很小。CD4细胞计数为200 - 500/mm³的患者增殖反应增强最为明显。有限稀释分析结果表明，IL-12存在时抗原特异性淋巴细胞增殖的增加是由于反应细胞数量的增加，而非固定数量反应细胞增殖程度的增加。
