Sabbaj S, Para M F, Fass R J, Adams P W, Orosz C G, Whitacre C C
Department of Medical Microbiology and Immunology, Ohio State University, Columbus 43210.
J Clin Immunol. 1992 May;12(3):216-24. doi: 10.1007/BF00918092.
The lymphocyte proliferative response to recall antigens is lost following HIV infection. We sought to devise a means by which the functional immune status of persons in the early stages of HIV infection could be monitored quantitatively. The response to tetanus toxoid was examined in 45 HIV-infected individuals and 11 controls using conventional lymphocyte proliferative assays concurrently with limiting dilution analysis utilizing the secretion of interleukin-2 as the measure of a response. Our data show that the limiting dilution analysis detects tetanus toxoid-reactive T cells in 80% of those tested, as compared to only 44% by proliferation. However, the frequency of tetanus-reactive T cells in HIV-infected individuals (median frequency = 1/59,156) is decreased five-fold as compared to seronegative controls (median frequency = 1/11,599). Longitudinal studies demonstrated a time-dependent decrease in the frequency of tetanus-specific T cell responses in the HIV-infected individuals. Thus, the limiting dilution analysis is a quantitative approach for detecting antigen-specific T cells in HIV-infected individuals, and may be used to monitor changes in T cell function in HIV infection.
HIV感染后，对回忆抗原的淋巴细胞增殖反应会丧失。我们试图设计一种方法，通过该方法可以定量监测HIV感染早期患者的功能性免疫状态。使用传统的淋巴细胞增殖试验，并同时采用以白细胞介素-2分泌作为反应指标的有限稀释分析，对45名HIV感染个体和11名对照者检测了对破伤风类毒素的反应。我们的数据表明，有限稀释分析在80%的受测者中检测到了破伤风类毒素反应性T细胞，而通过增殖检测仅为44%。然而，与血清阴性对照者（中位频率 = 1/11,599）相比，HIV感染个体中破伤风反应性T细胞的频率（中位频率 = 1/59,156）降低了五倍。纵向研究表明，HIV感染个体中破伤风特异性T细胞反应的频率呈时间依赖性下降。因此，有限稀释分析是检测HIV感染个体中抗原特异性T细胞的一种定量方法，可用于监测HIV感染中T细胞功能的变化。
