Lasry S, Dény P, Asselot C, Rauzy M, Boucher J, Guyot C, Leroux M C, Livartowski A, Reinert P, Nicolas J C
Department of Bacteriology and Virology, Avicenne Hospital, University Paris XIII, Bobigny, France.
J Infect Dis. 1996 Sep;174(3):606-9. doi: 10.1093/infdis/174.3.606.
Using the shell vial assay and sequence analysis of a variable region of the glycoprotein B (gB) gene, cytomegalovirus (CMV) excretion rates in urine and virus transmission were studied among 93 children from six day care centers (DCCs). During a 6-month period, excretion rates differed significantly between DCCs (P < .001). The 6 gB gene sequences, obtained from 24 CMV-infected children, were classified in four previously defined groups. In five DCCs, 2 or 3 strains cocirculated, and none was dominant. Infection could have been acquired outside the DCC for 2 children and inside it for 9. Two children from the same DCC had mixed infections. No differences in hygiene, child care practices, or experience and level of qualification of the staff could explain this wide variety of excretion rates between DCCs. The distribution of gB gene patterns observed does not suggest that 1 type was dominant or more efficiently transmitted.
采用空斑试验和糖蛋白B（gB）基因可变区序列分析，对来自6个日托中心（DCC）的93名儿童的尿中巨细胞病毒（CMV）排泄率及病毒传播情况进行了研究。在6个月的时间里，各日托中心的排泄率差异显著（P <.001）。从24名CMV感染儿童中获得的6个gB基因序列被归为先前定义的4组。在5个日托中心，有2或3种毒株同时流行，没有一种占主导地位。2名儿童可能是在日托中心外感染的，9名是在日托中心内感染的。来自同一日托中心的2名儿童有混合感染。卫生状况、儿童护理方式或工作人员的经验及资质水平的差异均无法解释各日托中心间如此大的排泄率差异。观察到的gB基因模式分布并不表明某一种类型占主导地位或传播更有效。
