Gonzalez-Sanchez Hilda M, Alvarado-Hernandez Diana L, Guerra-Palomares Sandra, Garcia-Sepulveda Christian A, Noyola Daniel E
Departamento de Bioquímica, Universidad Autónoma de San Luis Potosí, San Luis Potosí, México.
Intervirology. 2015;58(2):115-21. doi: 10.1159/000373922. Epub 2015 Mar 27.
Cytomegalovirus (CMV) is widely distributed and constitutes the main cause of congenital infections worldwide. CMV transmission during pregnancy represents one of the major impacts of this virus on public health. This study aimed at assessing glycoprotein B (gB) CMV genotypes in Mexican children and pregnant women, since there is limited information regarding CMV genomic diversity in Mexico.
We analyzed CMV strains detected in Mexican children (n = 38) and women (n = 38) between 2001 and 2012. A fragment of the gB gene was amplified and sequenced, and genotypes were defined based on prototype sequences.
The gB1 genotype was detected more frequently in children (68.4%) compared to women (31.6%; p = 0.0028), while genotype 2 was more common in women (65.8%) compared to children (26.3%, p = 0.0012). Genotype 3 was uncommon in both groups (5.3 and 2.6%). Nucleotide sequences exhibited a high degree of similarity to prototype strains. However, we identified 17 distinct sequences that resulted in changes in the encoded amino acid sequence in four strains.
gB1 and gB2 are the most common strains associated with CMV infection in Mexican children and women. In addition, we found that the frequency for each genotype differed amongst them, possibly due to variability in transmission or reactivation dynamics.
巨细胞病毒（CMV）广泛分布，是全球先天性感染的主要病因。孕期CMV传播是该病毒对公共卫生的主要影响之一。本研究旨在评估墨西哥儿童和孕妇中CMV糖蛋白B（gB）基因型，因为墨西哥关于CMV基因组多样性的信息有限。
我们分析了2001年至2012年间在墨西哥儿童（n = 38）和女性（n = 38）中检测到的CMV毒株。扩增并测序gB基因的一个片段，根据原型序列定义基因型。
与女性（31.6%；p = 0.0028）相比，gB1基因型在儿童中检出频率更高（68.4%），而基因型2在女性中更常见（65.8%），相比儿童（26.3%，p = 0.0012）。基因型3在两组中均不常见（5.3%和2.6%）。核苷酸序列与原型毒株表现出高度相似性。然而，我们鉴定出17个不同序列，导致4个毒株的编码氨基酸序列发生变化。
gB1和gB2是与墨西哥儿童和女性CMV感染相关的最常见毒株。此外，我们发现每种基因型的频率在他们之间存在差异，可能是由于传播或再激活动态的变异性。
