Wiese A, Reiners J O, Brandenburg K, Kawahara K, Zähringer U, Seydel U
Forschungsinstitut Borstel, Germany.
Biophys J. 1996 Jan;70(1):321-9. doi: 10.1016/S0006-3495(96)79573-5.
We have determined some physicochemical properties of the monosaccharide-type fraction (GSL-1) of glycosphingolipids, the major glycolipid components of the outer leaflet of the Gram-negative species Sphingomonas paucimobilis. These properties included the state of order of the hydrocarbon moiety, the effective molecular area, surface charge density, and intrinsic transmembrane potential profile of reconstituted planar asymmetric GSL-1/phospholipid bilayer membranes. We have, furthermore, investigated the insertion into and the function of porin channels in the reconstituted bilayers and the complement-activating capability of GSL-1 surfaces. All results were compared with respective data for deep rough mutant lipopolysaccharide of Salmonella minnesota R595. We found a remarkable agreement in most functional properties of the two glycolipids.
我们已经确定了糖鞘脂单糖型组分（GSL-1）的一些物理化学性质，糖鞘脂是革兰氏阴性菌少动鞘氨醇单胞菌外小叶的主要糖脂成分。这些性质包括烃部分的有序状态、有效分子面积、表面电荷密度以及重构的平面不对称GSL-1/磷脂双层膜的固有跨膜电位分布。此外，我们还研究了GSL-1在重构双层膜中插入孔蛋白通道的情况及其功能，以及GSL-1表面的补体激活能力。所有结果均与明尼苏达沙门氏菌R595深粗糙突变型脂多糖的相应数据进行了比较。我们发现这两种糖脂在大多数功能性质上有显著的一致性。
