平面膜中离子选择性孔蛋白的非线性和非对称开放通道特性。
Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes.
作者信息
Mathes A, Engelhardt H
机构信息
Max-Planck-Institut für Biochemie, Abteilung Molekulare Strukturbiologie, Martinsried, Germany.
出版信息
Biophys J. 1998 Sep;75(3):1255-62. doi: 10.1016/S0006-3495(98)74045-7.
Abstract
The open channel characteristics of the bacterial porin Omp32 from Comamonas acidovorans were investigated by means of conductance measurements in planar lipid bilayers of the Montal-Mueller type. Particularly at low salt conditions (< or = 30 mM KCl) Omp32 exhibited some unusual asymmetric and nonlinear functional properties. Current-voltage relationship measurements showed that conductance depends on the orientation of porin molecules and is a nonlinear function of the applied membrane potential. Conductance also depends on the salt concentration in a manner not common to porins and the salt concentration modulates the nonlinearity of conductance-voltage relationships. Omp32 is strongly anion-selective. The nonlinear and asymmetric conductance of the open channel is a new observation in porins.
摘要
通过在蒙塔尔-米勒型平面脂质双层中进行电导测量，研究了嗜酸丛毛单胞菌的细菌孔蛋白Omp32的开放通道特性。特别是在低盐条件下（≤30 mM KCl），Omp32表现出一些不寻常的不对称和非线性功能特性。电流-电压关系测量表明，电导取决于孔蛋白分子的取向，并且是施加膜电位的非线性函数。电导还以一种孔蛋白不常见的方式依赖于盐浓度，并且盐浓度调节电导-电压关系的非线性。Omp32具有很强的阴离子选择性。开放通道的非线性和不对称电导是孔蛋白中的一个新发现。
相似文献
1
Nonlinear and asymmetric open channel characteristics of an ion-selective porin in planar membranes.
Biophys J. 1998 Sep;75(3):1255-62. doi: 10.1016/S0006-3495(98)74045-7.
2
A voltage-dependent porin-like channel in the inner envelope membrane of plant chloroplasts.
J Biol Chem. 1995 Apr 28;270(17):9947-52. doi: 10.1074/jbc.270.17.9947.
3
Altered channel properties of porins from Haemophilus influenzae: isolates from cystic fibrosis patients.
J Membr Biol. 2002 Sep 15;189(2):131-41. doi: 10.1007/s00232-002-1008-6.
4
Single channel activity of OmpF-like porin from Yersinia pseudotuberculosis.
Biochim Biophys Acta. 2016 Apr;1858(4):883-91. doi: 10.1016/j.bbamem.2016.02.005. Epub 2016 Feb 17.
5
The cell wall porin of the gram-positive bacterium Nocardia asteroides forms cation-selective channels that exhibit asymmetric voltage dependence.
Arch Microbiol. 1999 Feb;171(3):173-82. doi: 10.1007/s002030050696.
6
Ion channels on synaptic vesicle membranes studied by planar lipid bilayer method.
Biophys J. 1992 Dec;63(6):1500-5. doi: 10.1016/S0006-3495(92)81731-9.
7
Crystal structure of Omp32, the anion-selective porin from Comamonas acidovorans, in complex with a periplasmic peptide at 2.1 A resolution.
Structure. 2000 Sep 15;8(9):981-92. doi: 10.1016/s0969-2126(00)00189-1.
8
Polarity-dependent voltage-gated porin channels from Escherichia coli in lipid bilayer membranes.
Biochim Biophys Acta. 1990 Jan 29;1021(2):175-81. doi: 10.1016/0005-2736(90)90031-i.
9
Single channel behavior of matrix porin of Escherichia coli.
Biochem Biophys Res Commun. 1993 Jan 29;190(2):624-9. doi: 10.1006/bbrc.1993.1094.
10
Rectification properties and pH-dependent selectivity of meningococcal class 1 porin.
Biophys J. 2008 Feb 15;94(4):1194-202. doi: 10.1529/biophysj.107.116186. Epub 2007 Oct 26.
引用本文的文献
1
Assessing the Effects of Surface-Stabilized Zero-Valent Iron Nanoparticles on Diverse Bacteria Species Using Complementary Statistical Models.
J Funct Biomater. 2025 Mar 20;16(3):113. doi: 10.3390/jfb16030113.
2
Gating of β-Barrel Protein Pores, Porins, and Channels: An Old Problem with New Facets.
Int J Mol Sci. 2023 Jul 28;24(15):12095. doi: 10.3390/ijms241512095.
3
Rectification properties and pH-dependent selectivity of meningococcal class 1 porin.
Biophys J. 2008 Feb 15;94(4):1194-202. doi: 10.1529/biophysj.107.116186. Epub 2007 Oct 26.
4
Molecular basis of bacterial outer membrane permeability revisited.
Microbiol Mol Biol Rev. 2003 Dec;67(4):593-656. doi: 10.1128/MMBR.67.4.593-656.2003.
5
Multistep mechanism of chloride translocation in a strongly anion-selective porin channel.
Biophys J. 2003 Aug;85(2):954-62. doi: 10.1016/S0006-3495(03)74534-2.
6
Electrostatic properties of the anion selective porin Omp32 from Delftia acidovorans and of the arginine cluster of bacterial porins.
Protein Sci. 2002 Jun;11(6):1309-19. doi: 10.1110/ps.4910102.
7
The regulated outer membrane protein Omp21 from Comamonas acidovorans is identified as a member of a new family of eight-stranded beta-sheet proteins by its sequence and properties.
J Bacteriol. 1998 Aug;180(15):3741-9. doi: 10.1128/JB.180.15.3741-3749.1998.
本文引用的文献
1
Crystallization and preliminary X-ray crystallographic studies of the native and chemically modified anion-selective porin from Comamonas acidovorans.
Acta Crystallogr D Biol Crystallogr. 1998 Jul 1;54(Pt 4):650-3. doi: 10.1107/s0907444997018854.
2
Disruption of polyamine modulation by a single amino acid substitution on the L3 loop of the OmpC porin channel.
Biochim Biophys Acta. 1997 Jun 12;1326(2):201-12. doi: 10.1016/s0005-2736(97)00024-2.
3
Voltage sensing in the PhoE and OmpF outer membrane porins of Escherichia coli: role of charged residues.
J Mol Biol. 1997 Jun 20;269(4):468-72. doi: 10.1006/jmbi.1997.1063.
4
1/f-Noise of open bacterial porin channels.
J Membr Biol. 1997 Jul 1;158(1):77-85. doi: 10.1007/s002329900245.
5
Voltage gating of Escherichia coli porin channels: role of the constriction loop.
Proc Natl Acad Sci U S A. 1997 Jun 24;94(13):6741-5. doi: 10.1073/pnas.94.13.6741.
6
Molecular cloning and functional characterization of the Paracoccus denitrificans porin.
Eur J Biochem. 1997 Apr 15;245(2):300-6. doi: 10.1111/j.1432-1033.1997.00300.x.
7
Electrophysiological characteristics of the PhoE porin channel from Escherichia coli. Implications for the possible existence of a superfamily of ion channels.
J Membr Biol. 1997 Mar 15;156(2):105-15. doi: 10.1007/s002329900193.
8
Permeation through an open channel: Poisson-Nernst-Planck theory of a synthetic ionic channel.
Biophys J. 1997 Jan;72(1):97-116. doi: 10.1016/S0006-3495(97)78650-8.
9
Structure and functional mechanism of porins.
Physiol Rev. 1996 Oct;76(4):1073-88. doi: 10.1152/physrev.1996.76.4.1073.
10
X-ray crystallographic and mass spectrometric structure determination and functional characterization of succinylated porin from Rhodobacter capsulatus: implications for ion selectivity and single-channel conductance.
Protein Sci. 1996 Aug;5(8):1477-89. doi: 10.1002/pro.5560050804.
Zotero 插件