Espinoza B, Parizade M, Ortega E, Tarrab-Hazdai R, Zilberg D, Arnon R
Departamento de Immunologia, Instituto de Investigaciones Biomédicas, Mexico D.F.
Hybridoma. 1995 Dec;14(6):577-86. doi: 10.1089/hyb.1995.14.577.
Monoclonal antibodies (MAbs) were raised in mice against acetylcholinesterase (AChE, EC 3.1.1.7) of the parasite Schistosoma mansoni. Specific tests were used, in which the hybridoma culture supernatants were screened for MAbs capable of recognizing AChE. The MAbs were characterized by their recognition of different stages of the parasite life cycle, by their binding to epitopes of protein or of carbohydrate, and by their capability of blocking AChE activity of the intact parasites. Furthermore, the MAbs were tested for their cross-reaction with AChE derived from various species. One of the MAbs, termed SA31, showed strong cross-reactivity with invertebrate and vertebrate species, indicating some similarity of cross-reaction between schistosome and mammalian AChE. However, most of the schistosome AChE epitopes are not shared with vertebrate AChE. The specific interaction of three other MAbs with intact schistosomula resulted in a marked complement (C)-dependent cytotoxicity. Specific schistosome AChE epitopes might be suitable candidates for drug design and vaccine preparation.
针对曼氏血吸虫寄生虫的乙酰胆碱酯酶（AChE，EC 3.1.1.7）在小鼠体内产生了单克隆抗体（MAb）。使用了特异性测试，其中对杂交瘤培养上清液进行筛选，以寻找能够识别AChE的单克隆抗体。这些单克隆抗体通过其对寄生虫生命周期不同阶段的识别、与蛋白质或碳水化合物表位的结合以及阻断完整寄生虫AChE活性的能力来进行表征。此外，还测试了这些单克隆抗体与源自各种物种的AChE的交叉反应。其中一种单克隆抗体，称为SA31，与无脊椎动物和脊椎动物物种表现出强烈的交叉反应，表明血吸虫和哺乳动物AChE之间存在一些交叉反应的相似性。然而，大多数血吸虫AChE表位与脊椎动物AChE并不共享。其他三种单克隆抗体与完整童虫的特异性相互作用导致了明显的补体（C）依赖性细胞毒性。特异性血吸虫AChE表位可能是药物设计和疫苗制备的合适候选物。
