Hamra J G, Yaksh T L
Department of Anesthesiology, University of California, San Diego, LaJolla 92093-0818, USA.
Immunopharmacol Immunotoxicol. 1996 May;18(2):323-36. doi: 10.3109/08923979609052739.
In order to determine the effects of halothane on rat cell-mediated immune function, rats were exposed to 1% halothane for up to 5 hours. Immediately, 24 hours or 48 hours following anesthesia, rat lymphocytes from the spleen were analyzed for their ability to respond to the mitogens phytohemagglutinin (PHA), pokeweed mitogen (PWM), concanavalin A (ConA) and lipopolysaccharide (LPS). In addition, percentages of lymphocyte subpopulations in the spleen were assessed as well as ability of the lymphocytes to express specific receptors. Extended periods of halothane anesthesia (5 hours) suppressed the ability of the lymphocytes to respond to the mitogen PHA immediately following anesthesia. Twenty-four hours later, proliferative responses to the mitogens PHA, PWM and ConA were significantly reduced. However, by 48 hours following treatment, proliferative responses were normal. Halothane did not alter proliferative responses to the mitogen LPS. Prolonged anesthesia (5 hours) also increased the percentage of T and CD8+ (cytotoxic) lymphocytes in the spleen, although for less than 24 hours. The ability of T lymphocytes to express both the CD8 and CD25 (IL-2) receptors in response to PHA were suppressed. These results suggest that halothane suppresses rat T cell function, perhaps through suppression of IL-2 receptor expression.
为了确定氟烷对大鼠细胞介导免疫功能的影响，将大鼠暴露于1%的氟烷中长达5小时。在麻醉后即刻、24小时或48小时，分析来自脾脏的大鼠淋巴细胞对有丝分裂原植物血凝素（PHA）、商陆有丝分裂原（PWM）、刀豆球蛋白A（ConA）和脂多糖（LPS）的反应能力。此外，评估脾脏中淋巴细胞亚群的百分比以及淋巴细胞表达特异性受体的能力。长时间的氟烷麻醉（5小时）在麻醉后即刻抑制了淋巴细胞对有丝分裂原PHA的反应能力。24小时后，对有丝分裂原PHA、PWM和ConA的增殖反应显著降低。然而，在处理后48小时，增殖反应恢复正常。氟烷并未改变对有丝分裂原LPS的增殖反应。长时间麻醉（5小时）也增加了脾脏中T淋巴细胞和CD8 +（细胞毒性）淋巴细胞的百分比，尽管持续时间不到24小时。T淋巴细胞对PHA反应时表达CD8和CD25（IL - 2）受体的能力受到抑制。这些结果表明，氟烷可能通过抑制IL - 2受体表达来抑制大鼠T细胞功能。
