Marakusha B I, Darwich K, Tartakovskiĭ I S
Zh Mikrobiol Epidemiol Immunobiol. 1996 May-Jun(3):60-4.
On the basis of specially developed scheme for the isolation of Listeria strains comprising 2 enrichment stages and the use of growth inhibitors, 128 L. monocytogenes cultures were isolated from clinical material, foodstuffs and sewage water. Highly virulent L.monocytogenes strains isolated from clinical material belonged to serovar 4b (54%) and 1/2a (38%), while those isolated from foodstuffs and sewage water belonged to 4b (74%). The restriction analysis of the chromosomal DNA of the isolated cultures with the use of restrictase EcoR1 on the basis of pulsed-field gel electrophoresis (PFGE) made it possible to distinguish Listeria strains in accordance with 5 types of restrictograms. The restrictograms of highly virulent L. monocytogenes strains, serovar 4b, belonged to types 1 and 2, while those of L. monocytogenes strains, serovar 1/2a, belonged to types 2 and 3. The comparative use of different methods for typing L. monocytogenes (sero-, phago-, bio- and resistotyping, the analysis of plasmid composition and restriction analysis) revealed that the combination of serotyping and restriction analysis on the basis of PFGE proved to be most promising for the characterization of the isolated L. monocytogenes strains and the assessment of their epidemic importance.
根据专门制定的用于分离李斯特菌菌株的方案，该方案包括2个富集阶段并使用生长抑制剂，从临床材料、食品和污水中分离出128株单核细胞增生李斯特菌培养物。从临床材料中分离出的高毒力单核细胞增生李斯特菌菌株属于血清型4b（54%）和1/2a（38%），而从食品和污水中分离出的菌株属于血清型4b（74%）。基于脉冲场凝胶电泳（PFGE），使用限制性内切酶EcoR1对分离出的培养物的染色体DNA进行限制性分析，使得能够根据5种限制性图谱类型区分李斯特菌菌株。高毒力单核细胞增生李斯特菌血清型4b菌株的限制性图谱属于类型1和2，而单核细胞增生李斯特菌血清型1/2a菌株的限制性图谱属于类型2和3。对单核细胞增生李斯特菌进行分型的不同方法（血清分型、噬菌体分型、生物分型和抗性分型、质粒组成分析和限制性分析）的比较使用表明，基于PFGE的血清分型和限制性分析相结合被证明对于鉴定分离出的单核细胞增生李斯特菌菌株及其流行重要性评估最有前景。
