Shaginian I A, Nesterenko L N, Grishina T D, Safonova S G, Golyshevskaia V I, Gintsburg A L, Khomenko A G, Prozorovskiĭ S V
Zh Mikrobiol Epidemiol Immunobiol. 1996 May-Jun(3):65-8.
Different genomic fingerprinting techniques (universal probes, such as rRNA genes, phage M13 DNA, IS 6110 probe) have been used to investigate the genomic polymorphism of Mycobacterium tuberculosis strains isolated in different geographical regions of Russia and in some CIS countries. As shown with the use of these techniques and a specially developed PCR-mediated system for genetic typing, M.tuberculosis strains are genotypically heterogeneous in regions with a sporadic level of tuberculosis morbidity and genotypically homogeneous in regions with elevated morbidity and mortality levels. The evaluation of the effectiveness of the genetic typing of M.tuberculosis with the use of different genomic fingerprinting techniques has made it possible to propose the optimum 3-stage scheme for the differentiation of M.tuberculosis strains: (1) the typing of all isolated strains of the PCR-mediated test system; (2) the typing of several selected M.tuberculosis strains with the use of 1S 6110 probe (2-3 strains of each detected PCR-RFLP [correction of PLRF] genotypes); (3) the typing of M.tuberculosis strains, containing 1 copy of 1S 6110 or not containing such sequence, with the use of probes (phage M13 DNA) detecting hypervariable sequences in M.tuberculosis genomes.
不同的基因组指纹识别技术（通用探针，如rRNA基因、噬菌体M13 DNA、IS 6110探针）已被用于研究在俄罗斯不同地理区域以及一些独联体国家分离出的结核分枝杆菌菌株的基因组多态性。使用这些技术以及专门开发的用于基因分型的PCR介导系统的研究表明，在结核病发病率呈散发性水平的地区，结核分枝杆菌菌株在基因型上具有异质性，而在发病率和死亡率较高的地区，结核分枝杆菌菌株在基因型上具有同质性。对使用不同基因组指纹识别技术进行结核分枝杆菌基因分型有效性的评估，使得提出区分结核分枝杆菌菌株的最佳三阶段方案成为可能：（1）对PCR介导检测系统中所有分离菌株进行分型；（2）使用IS 6110探针（每种检测到的PCR-RFLP[校正为PLRF]基因型的2-3株菌株）对几种选定的结核分枝杆菌菌株进行分型；（3）使用检测结核分枝杆菌基因组中高变序列的探针（噬菌体M13 DNA）对含有1个IS 6110拷贝或不含有此类序列的结核分枝杆菌菌株进行分型。
