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分枝杆菌参考单位日常工作中分子指纹识别策略的评估

Evaluation of strategies for molecular fingerprinting for use in the routine work of a Mycobacterium reference unit.

作者信息

Wilson S M, Goss S, Drobniewski F

机构信息

Public Health Laboratory Service Mycobacterium Reference Unit, Dulwich Hospital, East Dulwich Grove, East Dulwich, London SE22 8QF, United Kingdom.

出版信息

J Clin Microbiol. 1998 Nov;36(11):3385-8. doi: 10.1128/JCM.36.11.3385-3388.1998.

DOI:10.1128/JCM.36.11.3385-3388.1998
PMID:9774602
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC105338/
Abstract

We have investigated the role of two rapid PCR-based typing methods, IS6110-based PCR and spacer-oligonucleotide typing, within a national tuberculosis reference service. The validity of clusters with IS6110 restriction fragment length polymorphism fingerprints with less than 6 bands was also investigated in the context of referred isolates.

摘要

我们在一个国家结核病参考服务机构中研究了两种基于快速聚合酶链反应（PCR）的分型方法，即基于IS6110的PCR和间隔区寡核苷酸分型的作用。在送检分离株的背景下，还研究了IS6110限制性片段长度多态性指纹图谱中条带少于6条的聚类的有效性。

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Evaluation of strategies for molecular fingerprinting for use in the routine work of a Mycobacterium reference unit.分枝杆菌参考单位日常工作中分子指纹识别策略的评估
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本文引用的文献

1
Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence.从全基因组序列解读结核分枝杆菌的生物学特性。
Nature. 1998 Jun 11;393(6685):537-44. doi: 10.1038/31159.
2
Simultaneous detection and strain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology.同时检测结核分枝杆菌并进行菌株鉴别以用于诊断和流行病学研究。
J Clin Microbiol. 1997 Apr;35(4):907-14. doi: 10.1128/jcm.35.4.907-914.1997.
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DNA fingerprinting with two probes decreases clustering of Mycobacterium tuberculosis.使用两种探针进行DNA指纹识别可减少结核分枝杆菌的聚集。
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Evaluation of method for secondary DNA typing of Mycobacterium tuberculosis with pTBN12 in epidemiologic study of tuberculosis.在结核病流行病学研究中用pTBN12对结核分枝杆菌进行二级DNA分型的方法评估。
J Clin Microbiol. 1996 Dec;34(12):3044-8. doi: 10.1128/jcm.34.12.3044-3048.1996.
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Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains.用于结核分枝杆菌菌株IS6110指纹图谱分析的半巢式反向PCR
J Clin Microbiol. 1996 Jul;34(7):1686-90. doi: 10.1128/JCM.34.7.1686-1690.1996.
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Differentiation by molecular typing of Mycobacterium bovis strains causing tuberculosis in cattle and goats.通过对引起牛和山羊结核病的牛分枝杆菌菌株进行分子分型来鉴别。
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IS6110 based amplityping assay and RFLP fingerprinting of clinical isolates of Mycobacterium tuberculosis.基于IS6110的结核分枝杆菌临床分离株扩增分型检测及限制性片段长度多态性指纹分析
J Clin Pathol. 1995 Oct;48(10):924-8. doi: 10.1136/jcp.48.10.924.
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Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology.通过DNA指纹图谱技术进行结核分枝杆菌菌株鉴定：标准化方法建议
J Clin Microbiol. 1993 Feb;31(2):406-9. doi: 10.1128/jcm.31.2.406-409.1993.
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Rapid, simple method for typing isolates of Mycobacterium tuberculosis by using the polymerase chain reaction.
J Clin Microbiol. 1993 Feb;31(2):329-34. doi: 10.1128/jcm.31.2.329-334.1993.
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Rapid, amplification-based fingerprinting of Mycobacterium tuberculosis.结核分枝杆菌的快速、基于扩增的指纹识别
J Gen Microbiol. 1993 Jul;139(7):1537-42. doi: 10.1099/00221287-139-7-1537.