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通过连接介导的聚合酶链反应指纹分析快速鉴别结核分枝杆菌复合群菌株

Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.

作者信息

Prod'hom G, Guilhot C, Gutierrez M C, Varnerot A, Gicquel B, Vincent V

机构信息

Centre National de Référence des Mycobactéries, Institut Pasteur, Paris, France.

出版信息

J Clin Microbiol. 1997 Dec;35(12):3331-4. doi: 10.1128/jcm.35.12.3331-3334.1997.

DOI:10.1128/jcm.35.12.3331-3334.1997
PMID:9399550
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC230178/
Abstract

A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.

摘要

已开发出一种连接介导的PCR（LMPCR）方法，用于扩增结核分枝杆菌复合群IS6110侧翼的序列。该方法使用一种对IS6110特异的引物和另一种对连接到经SalI酶切的基因组DNA上的接头特异的引物。LMPCR是一种快速筛选方法，对结核分枝杆菌复合群菌株的指纹识别很有价值。

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本文引用的文献

1
Use of a PCR method based on IS6110 polymorphism for typing Mycobacterium tuberculosis strains from BACTEC cultures.基于IS6110多态性的PCR方法在对BACTEC培养的结核分枝杆菌菌株进行分型中的应用。
J Clin Microbiol. 1997 Jan;35(1):273-7. doi: 10.1128/jcm.35.1.273-277.1997.
2
Evaluation of method for secondary DNA typing of Mycobacterium tuberculosis with pTBN12 in epidemiologic study of tuberculosis.在结核病流行病学研究中用pTBN12对结核分枝杆菌进行二级DNA分型的方法评估。
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3
Spacer oligonucleotide typing of Mycobacterium bovis strains from cattle and other animals: a tool for studying epidemiology of tuberculosis.牛分枝杆菌菌株的间隔寡核苷酸分型：一种研究结核病流行病学的工具，来自牛和其他动物
J Clin Microbiol. 1996 Nov;34(11):2734-40. doi: 10.1128/jcm.34.11.2734-2740.1996.
4
DNA fingerprinting by infrequent-restriction-site amplification.通过稀有限制性酶切位点扩增进行DNA指纹分析。
J Clin Microbiol. 1996 Oct;34(10):2386-90. doi: 10.1128/jcm.34.10.2386-2390.1996.
5
Genotyping of Mycobacterium tuberculosis with additional markers enhances accuracy in epidemiological studies.采用额外标记对结核分枝杆菌进行基因分型可提高流行病学研究的准确性。
J Clin Microbiol. 1996 Sep;34(9):2219-24. doi: 10.1128/jcm.34.9.2219-2224.1996.
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Automated DNA fingerprinting analysis of Mycobacterium tuberculosis using fluorescent detection of PCR products.利用聚合酶链反应（PCR）产物的荧光检测对结核分枝杆菌进行自动化DNA指纹分析。
J Clin Microbiol. 1996 Jul;34(7):1801-3. doi: 10.1128/JCM.34.7.1801-1803.1996.
7
Heminested inverse PCR for IS6110 fingerprinting of Mycobacterium tuberculosis strains.用于结核分枝杆菌菌株IS6110指纹图谱分析的半巢式反向PCR
J Clin Microbiol. 1996 Jul;34(7):1686-90. doi: 10.1128/JCM.34.7.1686-1690.1996.
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Usefulness of the secondary probe pTBN12 in DNA fingerprinting of Mycobacterium tuberculosis.二级探针pTBN12在结核分枝杆菌DNA指纹分析中的实用性。
J Clin Microbiol. 1996 May;34(5):1118-23. doi: 10.1128/jcm.34.5.1118-1123.1996.
9
Evaluation of tuberculosis transmission in a community by 1 year of systematic typing of Mycobacterium tuberculosis clinical isolates.通过对结核分枝杆菌临床分离株进行为期1年的系统分型评估社区中的结核病传播情况。
J Clin Microbiol. 1996 May;34(5):1043-9. doi: 10.1128/jcm.34.5.1043-1049.1996.
10
DNA fingerprinting of Mycobacterium tuberculosis isolates by ligation-mediated polymerase chain reaction.通过连接介导的聚合酶链反应对结核分枝杆菌分离株进行DNA指纹分析。
Nucleic Acids Res. 1993 Feb 11;21(3):761-2. doi: 10.1093/nar/21.3.761.