通过连接介导的聚合酶链反应指纹分析快速鉴别结核分枝杆菌复合群菌株
Rapid discrimination of Mycobacterium tuberculosis complex strains by ligation-mediated PCR fingerprint analysis.
作者信息
Prod'hom G, Guilhot C, Gutierrez M C, Varnerot A, Gicquel B, Vincent V
机构信息
Centre National de Référence des Mycobactéries, Institut Pasteur, Paris, France.
出版信息
J Clin Microbiol. 1997 Dec;35(12):3331-4. doi: 10.1128/jcm.35.12.3331-3334.1997.
Abstract
A ligation-mediated PCR (LMPCR) method for the amplification of sequences flanking the IS6110 of the Mycobacterium tuberculosis complex has been developed. The method uses one primer specific for IS6110 and a second specific for a linker ligated to SalI-restricted genomic DNA. LMPCR is a rapid screening method, valuable for the fingerprinting of M. tuberculosis complex strains.
摘要
已开发出一种连接介导的PCR（LMPCR）方法，用于扩增结核分枝杆菌复合群IS6110侧翼的序列。该方法使用一种对IS6110特异的引物和另一种对连接到经SalI酶切的基因组DNA上的接头特异的引物。LMPCR是一种快速筛选方法，对结核分枝杆菌复合群菌株的指纹识别很有价值。
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本文引用的文献
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