Fujita M, Ikemoto M, Kishishita M, Otani H, Nohara R, Tanaka T, Tamaki S, Yamazato A, Sasayama S
College of Medical Technology, Kyoto University, Japan.
Circulation. 1996 Aug 15;94(4):610-3. doi: 10.1161/01.cir.94.4.610.
Collateral growth is induced by chemical signals from the ischemic myocardium. We hypothesized that angiogenic growth factors are produced by cardiac tissue; they are diffusible, more concentrated in pericardial fluids, and are increased by myocardial ischemia.
With the use of an enzyme-linked immunosorbent assay, we measured the concentrations of basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) in pericardial fluids of 12 patients with unstable angina (group 1) and of 8 patients with nonischemic heart diseases (group 2). The levels of protein in pericardial fluids were quite comparable between the two groups (34 +/- 2 versus 32 +/- 4 mg/mL). The concentration of bFGF in pericardial fluids in group 1 was 2036 +/- 357 pg/mL, significantly (P < .001) higher than the 289 +/- 72 pg/mL in group 2. The amount of bFGF per milligram of protein was also significantly (P < .05) higher in group 1 than in group 2 (67 +/- 15 versus 12 +/- 4 pg/mg). The concentration of VEGF in pericandial fluids tended to be higher in group 1, but the difference was statistically insignificant (39 +/- 7 versus 22 +/- 6 pg/mL). The amount of VEGF per milligram of protein was 1.2 +/- 0.3 pg/mg in group 1, similar to the 0.8 +/- 0.4 pg/mg in group 2.
This finding provides new evidence that bFGF plays an important role in mediating collateral growth in humans.
侧支循环的形成是由缺血心肌发出的化学信号所诱导的。我们推测血管生成生长因子由心脏组织产生；它们具有扩散性，在心包液中浓度更高，并且会因心肌缺血而增加。
我们采用酶联免疫吸附测定法，测量了12例不稳定型心绞痛患者（第1组）和8例非缺血性心脏病患者（第2组）心包液中碱性成纤维细胞生长因子（bFGF）和血管内皮生长因子（VEGF）的浓度。两组心包液中的蛋白质水平相当（34±2与32±4mg/mL）。第1组心包液中bFGF的浓度为2036±357pg/mL，显著高于第2组的289±72pg/mL（P<.001）。第1组每毫克蛋白质中bFGF的含量也显著高于第2组（P<.05）（67±15与12±4pg/mg）。第1组心包液中VEGF的浓度有升高趋势，但差异无统计学意义（39±7与22±6pg/mL）。第1组每毫克蛋白质中VEGF的含量为1.2±0.3pg/mg，与第2组的0.8±0.4pg/mg相似。
这一发现为bFGF在介导人类侧支循环形成中起重要作用提供了新证据。
