Mansour R T, Aboulghar M A, Serour G I, Fahmi I, Ramzy A M, Amin Y
Egyptian IVF-ET Center, Cairo, Egypt.
Fertil Steril. 1996 Mar;65(3):566-72. doi: 10.1016/s0015-0282(16)58155-2.
To evaluate treatment of couples who are infertile due to unreconstructable obstructive azoospermia with microsurgical epididymal sperm aspiration or testicular sperm and intracytoplasmic sperm injection (ICSI).
Prospective.
The Egyptian IVF-ET Center in association with Cairo University.
Twenty-three couples who are infertile due to obstructive azoospermia in which reconstructive microsurgery failed or was not possible.
Microsurgical epididymal or testicular sperm retrieval and ICSI in 24 cycles.
Fertilization and pregnancies.
All 24 cycles had successful fertilization and reached the ET stage. In microsurgical epididymal sperm aspiration cases, fertilization rate per metaphase II oocyte was 63% (109/ 172). Six patients became pregnant, including one set of twins and one set of triplets. One pregnancy resulted from the use of cryothawed epididymal sperm. In testicular sperm aspiration cases, the fertilization rate was 59% (32/54) and four clinical pregnancies resulted.
The use of microepididymal sperm aspiration or testicular sperm aspiration in conjunction with ICSI provide a highly precise and efficient procedure for achieving pregnancy in cases of unreconstuctable obstructive azoospermia.
评估采用显微附睾精子抽吸术或睾丸精子与卵胞浆内单精子注射（ICSI）治疗因梗阻性无精子症且无法重建而导致不育的夫妇的效果。
前瞻性研究。
埃及试管婴儿-胚胎移植中心与开罗大学联合。
23对因梗阻性无精子症导致不育的夫妇，其中重建显微手术失败或无法进行。
在24个周期中进行显微附睾或睾丸精子提取及ICSI。
受精和妊娠情况。
所有24个周期均成功受精并进入胚胎移植阶段。在显微附睾精子抽吸术病例中，每个中期II级卵母细胞的受精率为63%（109/172）。6名患者怀孕，包括1例双胞胎和1例三胞胎。1例妊娠是使用冷冻复苏的附睾精子所致。在睾丸精子抽吸术病例中，受精率为59%（32/54），有4例临床妊娠。
对于无法重建的梗阻性无精子症病例，联合使用显微附睾精子抽吸术或睾丸精子抽吸术与ICSI为实现妊娠提供了一种高度精确且有效的方法。
