卵胞浆内单精子注射后精子活力对受精和胚胎分裂的影响。
Effects of sperm viability on fertilization and embryo cleavage following intracytoplasmic sperm injection.
作者信息
Poe-Zeigler R, Nehchiri F, Hamacher P, Boyd C, Oehninger S, Muasher S, Lanzendorf S E
机构信息
Jones Institute for Reproductive Medicine, Department of Obstetrics and Gynecology, Eastern Virginia Medical School, Norfolk 23507, USA.
出版信息
J Assist Reprod Genet. 1997 May;14(5):277-81. doi: 10.1007/BF02765829.
PURPOSE
In the human, intracytoplasmic sperm injection is typically performed using "viable" sperm which has been mechanically rendered nonmotile. The purpose of the present study was to determine the ability of nonviable sperm to fertilize human oocytes and the early developmental normalcy of the resulting embryos.
METHODS
In this study, immature, prophase I oocytes from a total of 27 consenting patients were matured in vitro and then randomized into two groups: injection with a viable human sperm or injection with a sperm rendered nonviable by freeze-thawing in liquid nitrogen. The rates of fertilization and cleavage were compared between the two groups.
RESULTS
The results demonstrated a significantly higher two-pronuclear fertilization rate when oocytes were injected with viable sperm (62.2%) compared to when oocytes were injected with nonviable sperm (16.2%). Oocytes injected with viable sperm also demonstrated a higher cleavage rate (91 vs 33%).
CONCLUSIONS
These findings suggest that while the intracytoplasmic injection of nonviable human sperm can result in normal fertilization, it does so at a much reduced rate compared to viable sperm and may not result in normally cleaving embryos.
目的
在人类中，胞浆内单精子注射通常使用经机械处理而失去活力的“活”精子进行。本研究的目的是确定无活力精子使人类卵母细胞受精的能力以及所产生胚胎的早期发育正常情况。
方法
在本研究中，从总共27名同意参与的患者中获取未成熟的前期I卵母细胞，在体外使其成熟，然后随机分为两组：注射活的人类精子或注射经液氮冻融处理而失去活力的精子。比较两组的受精率和卵裂率。
结果
结果表明，与注射无活力精子的卵母细胞（16.2%）相比，注射活精子的卵母细胞的双原核受精率显著更高（62.2%）。注射活精子的卵母细胞的卵裂率也更高（分别为91%和33%）。
结论
这些发现表明，虽然胞浆内注射无活力的人类精子可以导致正常受精，但与活精子相比，其受精率大大降低，并且可能不会产生正常卵裂的胚胎。
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