Ramey J W, Seltman H J, Toner J P
Jones Institute for Reproductive Medicine, Department of Obsetrics and Gynecology, Eastern Virginia Medical School, Norfolk, USA.
Fertil Steril. 1996 Mar;65(3):661-3. doi: 10.1016/s0015-0282(16)58172-2.
To determine whether an analytically superior gonadotropin assay, the monoclonal, two-site immunometric assay, also provided superior prediction of clinical outcomes when compared with conventional RIA methodology.
Methods comparison study.
Tertiary academic center.
One hundred fifty-seven consecutive IVF patients.
Comparisons of FSH and LH levels on cycle day 3 were made using paired RIA and immunometric assay procedures. The ability of day 3 LH, FSH, and their ratio in predicting IVF performance was determined using regression analyses.
The predictive ability of FSH as assayed by immunometric assay at least equaled that obtained by RIA for both peak E2 levels and the number of mature oocytes retrieved.
Results from this study indicate that gonadotropin levels as measured by immunometric assay represent an effective clinical tool in predicting IVF outcomes that may prove superior to RIA.
确定一种分析性能更优的促性腺激素检测方法，即单克隆双位点免疫测定法，与传统放射免疫分析（RIA）方法相比，在预测临床结局方面是否也更具优势。
方法比较研究。
三级学术中心。
157例连续的体外受精（IVF）患者。
采用配对的RIA和免疫测定法程序比较第3天周期的促卵泡激素（FSH）和促黄体生成素（LH）水平。使用回归分析确定第3天的LH、FSH及其比值预测IVF表现的能力。
免疫测定法检测的FSH在预测峰值雌二醇（E2）水平和回收的成熟卵母细胞数量方面，其预测能力至少与RIA法相当。
本研究结果表明，免疫测定法测得的促性腺激素水平是预测IVF结局的一种有效临床工具，可能比RIA法更具优势。
