Figueiredo B C, Plüss K, Skup M, Otten U, Cuello A C
Department of Pharmacology and Therapeutics, McGill University, Montreal, Que., Canada.
Brain Res Mol Brain Res. 1995 Oct;33(1):1-6. doi: 10.1016/0169-328x(95)00099-e.
Recently we reported that human recombinant acidic fibroblast growth factor (aFGF) is capable of preventing degeneration of nucleus basalis magnocellularis neurons in vivo and inducing growth of astrocytes in vitro. In the present study, the effects of aFGF on the concentration of nerve growth factor (NGF) and its messenger RNA were investigated in the rat cerebral cortex following unilateral cortical infarction. Lesioned animals exhibited a significant increase of NGF in the remaining cortex ipsilateral to the lesion. After combining cortical lesion with intracerebroventricular application of aFGF (12 micrograms/day for 7 days), we observed an 8-fold increase in the NGF concentration and a marked increase in the level of steady state NGF mRNA relative to controls ipsilaterally, and a less pronounced aFGF effect in the contralateral cerebral cortex. These results support the hypothesis that the neurotrophic effects previously shown for aFGF and basic FGF (bFGF) in neurotrophin-sensitive neurons is mediated by inducing increased production of NGF within the injured central nervous system (CNS) of adult animals.
最近我们报道，人重组酸性成纤维细胞生长因子（aFGF）能够在体内预防基底核大细胞神经元的退变，并在体外诱导星形胶质细胞生长。在本研究中，我们研究了aFGF对单侧皮质梗死大鼠大脑皮质中神经生长因子（NGF）浓度及其信使核糖核酸的影响。受损动物在损伤同侧的剩余皮质中NGF显著增加。在将皮质损伤与脑室内应用aFGF（12微克/天，共7天）相结合后，我们观察到同侧相对于对照组，NGF浓度增加了8倍，稳态NGF mRNA水平显著升高，而对侧大脑皮质中aFGF的作用则不太明显。这些结果支持以下假说：先前在神经营养因子敏感神经元中显示的aFGF和碱性成纤维细胞生长因子（bFGF）的神经营养作用是通过诱导成年动物受损中枢神经系统（CNS）内NGF产生增加来介导的。
