Nelson J E, Krawetz S A
Department of Obstetrics and Gynecology, Wayne State University School of Medicine, Detroit, MI 48201, USA.
DNA Seq. 1995;5(6):329-37. doi: 10.3109/10425179509020864.
The promoter regions of the clustered human spermatid-specific nucleoprotein PRM1, PRM2 arid TNP2 genes were compared to define regulatory elements that may govern their expression. Sequence alignment revealed two wll conserved motifs, despite a lack of extensive homology. They are located at similar positions within the first 400 nt of their 5' UTRs. Conservation of these motifs may reflect selective evolutionary pressure to maintain their structure. This supports the view that these elements assume a central role in the coordinate regulation of this gene cluster during spermiogenesis. The distribution of binding sites of known transcription factors was also assessed within the regions flanking the 5' ends of these genes. This analysis should prove useful in directing studies that define the signals necessary for the coordinate regulation of this spermatid specific gene cluster.
对成簇的人类精子细胞特异性核蛋白PRM1、PRM2和TNP2基因的启动子区域进行比较，以确定可能调控其表达的调控元件。序列比对显示，尽管缺乏广泛的同源性，但存在两个高度保守的基序。它们位于其5'非翻译区前400个核苷酸内的相似位置。这些基序的保守性可能反映了维持其结构的选择性进化压力。这支持了这样一种观点，即这些元件在精子发生过程中对该基因簇的协调调控中起核心作用。还评估了这些基因5'端侧翼区域内已知转录因子结合位点的分布。该分析对于指导确定该精子细胞特异性基因簇协调调控所需信号的研究应是有用的。
