He M, Xu J, Chan T W, Lau R L
Department of Chemistry, Peking University, Beijing, P.R. China.
Rapid Commun Mass Spectrom. 1996;10(8):897-902. doi: 10.1002/(SICI)1097-0231(19960610)10:8<897::AID-RCM527>3.0.CO;2-G.
We have determined the mass spectra of three polypeptides which have been designed as an effective reagent to be used in the diagnosis of a Chinese strain of Hepatitis E Virus (HEV). The spectra were determined by electrospray ionization-Fourier transform ion cyclotron resonance mass spectrometer. These are peptide 30, peptide 33 and peptide 42, where the guanidine amino group of arginine in each polypeptide was protected. The molecular weights were respectively measured as 3575, 4094 and 5390 Dalton, against the molecular weights of bovine serum albumin (66430 Da) and bovine insulin (5732.77 Da).
我们已经测定了三种多肽的质谱，这些多肽被设计为一种有效的试剂，用于诊断中国株戊型肝炎病毒（HEV）。这些谱图是通过电喷雾电离-傅里叶变换离子回旋共振质谱仪测定的。它们是肽30、肽33和肽42，其中每种多肽中精氨酸的胍基氨基都受到了保护。相对于牛血清白蛋白（66430道尔顿）和牛胰岛素（5732.77道尔顿）的分子量，分别测得分子量为3575、4094和5390道尔顿。
