Gehring H, Nahm W, Klotz K F, Zais O, Schreiber R, Schüren P, Schmucker P
Klinik für Anaesthesiologie, Medizinischen Universität Lübeck.
Infusionsther Transfusionsmed. 1996 Apr;23(2):86-91.
The determination of the current intravascular plasma volume with the dye dilution method is of major interest in therapeutic intervention and scientific research. The use of the rapidly eliminated dye indocyanine green (ICG) allows to perform plasma volume measurements serially and to observe a time course. The following questions should be investigated in the present study: 1. Do the plasma volume (PV) values measured by the ICG method in this study agree with data of other authors with special respect to basic values for further clinical investigations, and 2. Do the measured differences coincide with the withdrawn and retransfused plasma volume?
Prospective study (on each 20 healthy female and male volunteers).
Research laboratory of a university department of anesthesiology.
Each 20 healthy female and male volunteers.
Measurement of PV with ICG both before and after withdrawal of 10% of blood volume and after retransfusion.
The plasma volume per body surface area (PV/BSA) was in the group of female volunteers 1639 +/- 198 and in male volunteers 1687 +/- 224 ml/m2 (mean +/- SD). The withdrawn amount of plasma volume (EPV) was 188 +/- 23 in the female group and 149 +/- 26 ml/m2 in the male group. Determined by the ICG method the difference for the withdrawal was in female volunteers 198 +/- 174 and in male volunteers 171 +/- 158 ml/m2 and for the retransfusion in the female group 190 +/- 169 and in the male group 142 +/- 154 ml/m2.
The mean differences in plasma volume measured by the ICG method both before and after withdrawal and after retransfusion of autologous blood were in good agreement with the withdrawn amount of plasma volume. The large standard deviation has to be considered in clinical assessment of an individual measurement.
采用染料稀释法测定当前血管内血浆容量在治疗干预和科学研究中具有重要意义。使用快速消除的染料吲哚菁绿（ICG）能够连续进行血浆容量测量并观察其时间进程。本研究应探讨以下问题：1. 本研究中通过ICG法测量的血浆容量（PV）值与其他作者的数据相比，在进一步临床研究的基础值方面是否一致；2. 测量的差异是否与抽取和回输的血浆容量一致？
前瞻性研究（对20名健康男女志愿者进行）。
大学麻醉学系研究实验室。
20名健康男女志愿者。
在抽取10%血容量前后以及回输后，用ICG测量PV。
女性志愿者组每体表面积的血浆容量（PV/BSA）为1639±198，男性志愿者组为1687±224 ml/m²（均值±标准差）。女性组抽取的血浆容量（EPV）为188±23，男性组为149±26 ml/m²。通过ICG法测定，女性志愿者抽取时的差异为198±174，男性志愿者为171±158 ml/m²；女性组回输时的差异为190±169，男性组为142±154 ml/m²。
自体血抽取前后及回输后，通过ICG法测量的血浆容量平均差异与抽取的血浆容量高度一致。在对个体测量进行临床评估时，必须考虑较大的标准差。
