Rangel D M, Golub S H, Morton D L
Surgery. 1977 Aug;82(2):224-32.
The proliferative response of lymphocytes obtained from a healthy volunteer to the mitogens phytohemagglutinin (PHA) and concanavalin A (Con A) was evaluated in the presence of sera from 101 patients with malignant melanoma and 75 normal controls. A significant decrease in blastogenic response was observed in the presence of sera from melanoma patients. Sera from patients with advanced disease were particularly inhibitory, suggesting that the circulating immunosuppressive factors were either tumor derived or tumor induced. Our observation that sera from patients who failed to display a delayed cutaneous hypersensitivity reaction to 2,4-dinitrochlorobenzene (DNCB) are more inhibitory to mitogen stimulation than sera from patients who react to this contact allergen suggests that DNCB skin testing may identify an immunological defect mediated by circulating humoral factors.
在来自101例恶性黑色素瘤患者的血清和75例正常对照血清存在的情况下，评估了从一名健康志愿者获得的淋巴细胞对丝裂原植物血凝素（PHA）和刀豆球蛋白A（Con A）的增殖反应。在黑色素瘤患者血清存在的情况下，观察到母细胞形成反应显著降低。晚期疾病患者的血清具有特别的抑制作用，这表明循环免疫抑制因子要么是肿瘤来源的，要么是肿瘤诱导的。我们的观察结果表明，对2,4-二硝基氯苯（DNCB）未表现出迟发性皮肤超敏反应的患者血清，比对这种接触性变应原产生反应的患者血清对丝裂原刺激的抑制作用更强，这表明DNCB皮肤试验可能识别出由循环体液因子介导的免疫缺陷。
