Chang D, Yi S J, Baram T Z
Department of Pediatrics, University of California at Irvine 92717, USA.
Int J Dev Neurosci. 1996 Feb;14(1):69-76. doi: 10.1016/0736-5748(95)00072-0.
Corticotropin releasing hormone is a neurotransmitter in the inferior olive complex of marsupials and mammals. The ontogeny of corticotropin releasing hormone gene expression in the rat inferior olive has not been described. Using in-situ hybridization histochemistry in 25 animals, we established the developmental profile of the peptide's messenger ribonucleic acid in the postnatal rat. CRH-messenger RNA was first detectable in two of four animals on the fifth postnatal day. Subsequently, gene expression increased linearly with age: by day 14, CRH was expressed in all olivary complex nuclei, and the distribution and relative abundance on day 18 were comparable to those in the adult. The developmental profile of CRH-mRNA in the rat inferior olive differs from those in the mouse and opossum, and from the pattern in the rat hypothalamus, suggesting species- and site-specificity of the peptide's function.
促肾上腺皮质激素释放激素是有袋动物和哺乳动物下橄榄复合体中的一种神经递质。大鼠下橄榄中促肾上腺皮质激素释放激素基因表达的个体发生尚未见报道。我们利用25只动物的原位杂交组织化学技术，确定了该肽的信使核糖核酸在出生后大鼠中的发育情况。促肾上腺皮质激素释放激素信使核糖核酸在出生后第5天在4只动物中的2只中首次可检测到。随后，基因表达随年龄呈线性增加：到第14天，促肾上腺皮质激素释放激素在所有橄榄复合体核中表达，第18天的分布和相对丰度与成年动物相当。大鼠下橄榄中促肾上腺皮质激素释放激素信使核糖核酸的发育情况与小鼠和负鼠不同，也与大鼠下丘脑的模式不同，这表明该肽功能具有物种和位点特异性。
