Zhong M, Zhou J, Lunte S M, Zhao G, Giolando D M, Kirchhoff J R
Center for Bioanalytical Research, University of Kansas, Lawrence 66047, USA.
Anal Chem. 1996 Jan 1;68(1):203-7. doi: 10.1021/ac950545p.
The extremely low sample volumes required for capillary electrophoresis and the high sensitivity and selectivity of electrochemical detection make capillary electrophoresis/electrochemistry (CEEC) a very useful method for bioanalysis. In this paper, two types of dual-electrode detectors for CEEC are described. The first employs a ring-disk microelectrode placed in a wall-jet configuration and is used for the selective detection of substances undergoing chemically reversible oxidations. Collection efficiencies obtained for catecholamines with this configuration were between 25 and 35%. The second electrode design consists of two adjacent carbon fibers embedded in an epoxy matrix and is analogous to the parallel dual-electrode configuration used in liquid chromatography/electrochemistry. This configuration can be used to confirm peak identity and purity by operating the electrodes at two different potentials. Alternatively, it is possible to perform simultaneous oxidative and reductive electrochemical detection.
毛细管电泳所需的极低样品体积以及电化学检测的高灵敏度和选择性，使得毛细管电泳/电化学（CEEC）成为生物分析中一种非常有用的方法。本文描述了两种用于CEEC的双电极检测器。第一种采用置于壁流配置中的环盘微电极，用于选择性检测发生化学可逆氧化的物质。采用这种配置对儿茶酚胺获得的收集效率在25%至35%之间。第二种电极设计由嵌入环氧树脂基质中的两根相邻碳纤维组成，类似于液相色谱/电化学中使用的平行双电极配置。通过在两个不同电位下操作电极，这种配置可用于确认峰的同一性和纯度。或者，也可以进行同时的氧化和还原电化学检测。
