Voegel P D, Baldwin R P
Department of Chemistry, University of Louisville, KY 40292, USA.
Electrophoresis. 1998 Sep;19(12):2226-32. doi: 10.1002/elps.1150191230.
A new approach for dual electrode electrochemical detection in capillary electrophoresis (CEEC) is described. In this approach, two identical capillaries, each containing an on-capillary electrode incorporated permanently onto its tip, were paired together for simultaneous sample injection and detection. This procedure permitted dual-parallel detection to be performed without the need for painstaking alignment of the electrodes with respect to one another and to the capillary outlet as is required for the off-capillary microelectrode systems usually employed in CEEC. As a result, independent detection at two electrodes held at different potentials or at two electrodes of different composition or structure could be performed simply and with wide flexibility. Fabrication of on-capillary electrodes was carried out by sputter-coating the exit end of the capillaries with a thin layer of Au or Pt. Dual electrode system performance was demonstrated by separation and analysis of phenol and catechol samples. In addition, the detection system was coupled with glucose oxidase for the selective detection of glucose.
描述了一种用于毛细管电泳（CEEC）中双电极电化学检测的新方法。在这种方法中，两根相同的毛细管，每根毛细管的尖端都永久地结合有一个毛细管内电极，将它们配对在一起用于同时进样和检测。该过程允许进行双平行检测，而无需像CEEC中通常使用的毛细管外微电极系统那样，费力地将电极彼此之间以及相对于毛细管出口进行对齐。结果，可以简单且灵活地在保持不同电位的两个电极或不同组成或结构的两个电极上进行独立检测。通过在毛细管的出口端溅射涂覆一层薄的金或铂来制造毛细管内电极。通过对苯酚和邻苯二酚样品的分离和分析，证明了双电极系统的性能。此外，该检测系统与葡萄糖氧化酶联用，用于葡萄糖的选择性检测。
