乳酸乳球菌乳酸亚种双乙酰变种突变体过量产生双乙酰和α-乙酰乳酸，这些突变体缺乏α-乙酰乳酸脱羧酶且乳酸脱氢酶活性较低。
Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activity.
作者信息
Monnet C, Aymes F, Corrieu G
机构信息
Laboratoire de Génie et Microbiologie des Procédés Alimentaires, Institut National de la Recherche Agronomique, 78850 Thiverval-Grignon, France.
出版信息
Appl Environ Microbiol. 2000 Dec;66(12):5518-20. doi: 10.1128/AEM.66.12.5518-5520.2000.
Abstract
Lactococcus lactis subsp. lactis biovar diacetylactis strains are utilized in several industrial processes for producing the flavoring compound diacetyl or its precursor alpha-acetolactate. Using random mutagenesis with nitrosoguanidine, we selected mutants that were deficient in alpha-acetolactate decarboxylase and had low lactate dehydrogenase activity. The mutants produced large amounts of alpha-acetolactate in anaerobic milk cultures but not in aerobic cultures, except when the medium was supplemented with catalase, yeast extract, or hemoglobin.
摘要
乳酸乳球菌乳酸亚种双乙酰变种菌株被用于多个工业生产过程，以生产调味化合物双乙酰或其前体α-乙酰乳酸。利用亚硝基胍进行随机诱变，我们筛选出了α-乙酰乳酸脱羧酶缺陷且乳酸脱氢酶活性较低的突变体。这些突变体在厌氧的牛奶培养物中会产生大量α-乙酰乳酸，但在需氧培养物中则不会，除非培养基中添加了过氧化氢酶、酵母提取物或血红蛋白。
相似文献
1
Diacetyl and alpha-acetolactate overproduction by Lactococcus lactis subsp. lactis biovar diacetylactis mutants that are deficient in alpha-acetolactate decarboxylase and have a low lactate dehydrogenase activity.乳酸乳球菌乳酸亚种双乙酰变种突变体过量产生双乙酰和α-乙酰乳酸，这些突变体缺乏α-乙酰乳酸脱羧酶且乳酸脱氢酶活性较低。
Appl Environ Microbiol. 2000 Dec;66(12):5518-20. doi: 10.1128/AEM.66.12.5518-5520.2000.
2
Glucose/citrate cometabolism in Lactococcus lactis subsp. lactis biovar diacetylactis with impaired alpha-acetolactate decarboxylase.乳酸乳球菌乳酸亚种双乙酰变种中α-乙酰乳酸脱羧酶受损时的葡萄糖/柠檬酸盐共代谢
Metab Eng. 1999 Oct;1(4):291-8. doi: 10.1006/mben.1999.0123.
3
Imbalance of leucine flux in Lactococcus lactis and its use for the isolation of diacetyl-overproducing strains.乳酸乳球菌中亮氨酸通量的失衡及其在筛选双乙酰高产菌株中的应用。
Appl Environ Microbiol. 1996 Jul;62(7):2636-40. doi: 10.1128/aem.62.7.2636-2640.1996.
4
Effect of alpha-acetolactate decarboxylase inactivation on alpha-acetolactate and diacetyl production by Lactococcus lactis subsp. lactis biovar diacetylactis.α-乙酰乳酸脱羧酶失活对乳酸乳球菌乳亚种双乙酰变种产生α-乙酰乳酸和双乙酰的影响。
J Biosci Bioeng. 1999;87(1):87-92. doi: 10.1016/s1389-1723(99)80013-9.
5
A general method for selection of alpha-acetolactate decarboxylase-deficient Lactococcus lactis mutants to improve diacetyl formation.一种筛选α-乙酰乳酸脱羧酶缺陷型乳酸乳球菌突变体以提高双乙酰生成量的通用方法。
Appl Environ Microbiol. 1999 Mar;65(3):1202-6. doi: 10.1128/AEM.65.3.1202-1206.1999.
6
Selection and properties of alpha-acetolactate decarboxylase-deficient spontaneous mutants of Streptococcus thermophilus.嗜热链球菌α-乙酰乳酸脱羧酶缺陷型自发突变体的筛选与特性
Food Microbiol. 2007 Sep;24(6):601-6. doi: 10.1016/j.fm.2007.01.004. Epub 2007 Jan 14.
7
Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and acetoin from glucose.乳酸乳球菌乳酸亚种双乙酰变种CNRZ 483从葡萄糖产生双乙酰和乙偶姻的突变体的分离及特性
Appl Environ Microbiol. 1997 Jun;63(6):2293-9. doi: 10.1128/aem.63.6.2293-2299.1997.
8
Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis.乳酸乳球菌中双乙酰代谢途径的基因操作。
Appl Environ Microbiol. 1996 Jul;62(7):2641-3. doi: 10.1128/aem.62.7.2641-2643.1996.
9
Lactococcus lactis as a cell factory for high-level diacetyl production.乳酸乳球菌作为用于高水平双乙酰生产的细胞工厂。
Appl Environ Microbiol. 2000 Sep;66(9):4112-4. doi: 10.1128/AEM.66.9.4112-4114.2000.
10
Thiamine-Starved for Producing Food-Grade Pyruvate.生产食品级丙酮酸的硫胺素饥饿
J Agric Food Chem. 2024 Mar 6;72(9):4858-4868. doi: 10.1021/acs.jafc.3c09216. Epub 2024 Feb 20.
引用本文的文献
1
Citrate metabolism in lactic acid bacteria: is there a beneficial effect for in wine?乳酸菌中的柠檬酸盐代谢：对葡萄酒有有益影响吗？
Front Microbiol. 2024 Jan 4;14:1283220. doi: 10.3389/fmicb.2023.1283220. eCollection 2023.
2
Isolation of ssp. LRCC5306 and Optimization of Diacetyl Production Conditions for Manufacturing Sour Cream.嗜柠檬酸明串珠菌亚种LRCC5306的分离及用于生产酸奶油的双乙酰生产条件优化
Food Sci Anim Resour. 2021 May;41(3):373-385. doi: 10.5851/kosfa.2021.e3. Epub 2021 May 1.
3
Development of Biosensors for Detection of Diacetyl.用于检测双乙酰的生物传感器的开发。
Front Microbiol. 2020 May 25;11:1032. doi: 10.3389/fmicb.2020.01032. eCollection 2020.
4
Efficient production of α-acetolactate by whole cell catalytic transformation of fermentation-derived pyruvate.利用发酵衍生的丙酮酸进行全细胞催化转化生产 α-乙酰乳酸。
Microb Cell Fact. 2019 Dec 29;18(1):217. doi: 10.1186/s12934-019-1271-1.
5
Systems Biology - A Guide for Understanding and Developing Improved Strains of Lactic Acid Bacteria.系统生物学——理解与开发改良乳酸菌菌株指南
Front Microbiol. 2019 Apr 30;10:876. doi: 10.3389/fmicb.2019.00876. eCollection 2019.
6
Effect of Respiratory Growth on the Metabolite Production and Stress Robustness of N87 Cultivated in Cheese Whey Permeate Medium.呼吸生长对在奶酪乳清渗透液培养基中培养的N87代谢产物产生及应激抗性的影响。
Front Microbiol. 2019 Apr 24;10:851. doi: 10.3389/fmicb.2019.00851. eCollection 2019.
7
Metabolic Engineering of Fusarium oxysporum to Improve Its Ethanol-Producing Capability.尖孢镰刀菌的代谢工程改造以提高其乙醇生产能力
Front Microbiol. 2016 May 4;7:632. doi: 10.3389/fmicb.2016.00632. eCollection 2016.
8
Identifying Potential Mechanisms Enabling Acidophily in the Ammonia-Oxidizing Archaeon "Candidatus Nitrosotalea devanaterra".确定氨氧化古菌“暂定硝化螺菌属德瓦纳特拉菌”嗜酸特性的潜在机制
Appl Environ Microbiol. 2016 Apr 18;82(9):2608-2619. doi: 10.1128/AEM.04031-15. Print 2016 May.
9
Production of diacetyl by metabolically engineered Enterobacter cloacae.通过代谢工程改造的阴沟肠杆菌生产双乙酰。
Sci Rep. 2015 Mar 12;5:9033. doi: 10.1038/srep09033.
10
Metabolic engineering of Candida glabrata for diacetyl production.用于双乙酰生产的光滑念珠菌的代谢工程。
PLoS One. 2014 Mar 10;9(3):e89854. doi: 10.1371/journal.pone.0089854. eCollection 2014.
本文引用的文献
1
A colorimetric determination of blood acetoin.血液中乙偶姻的比色测定法。
J Biol Chem. 1945 Dec;161:495-502.
2
Development and Use of a Screening Procedure for Production of (alpha)-Acetolactate by Lactococcus lactis subsp. lactis biovar diacetylactis Strains.开发和使用筛选程序生产（α）-乙酰乳酸的乳球菌乳亚种。双乙酰亚种菌株。
Appl Environ Microbiol. 1997 Feb;63(2):793-5. doi: 10.1128/aem.63.2.793-795.1997.
3
Improved medium for lactic streptococci and their bacteriophages.用于乳酸链球菌及其噬菌体的改良培养基。
Appl Microbiol. 1975 Jun;29(6):807-13. doi: 10.1128/am.29.6.807-813.1975.
4
Study of the Citrate Metabolism of Lactococcus lactis subsp. lactis Biovar Diacetylactis by Means of C Nuclear Magnetic Resonance.采用 C 核磁共振研究乳球菌乳亚种丁二酮变种的柠檬酸盐代谢。
Appl Environ Microbiol. 1991 Nov;57(11):3371-7. doi: 10.1128/aem.57.11.3371-3377.1991.
5
Effect of alpha-acetolactate decarboxylase inactivation on alpha-acetolactate and diacetyl production by Lactococcus lactis subsp. lactis biovar diacetylactis.α-乙酰乳酸脱羧酶失活对乳酸乳球菌乳亚种双乙酰变种产生α-乙酰乳酸和双乙酰的影响。
J Biosci Bioeng. 1999;87(1):87-92. doi: 10.1016/s1389-1723(99)80013-9.
6
Method for the selection of Lactococcus lactis mutants producing excess carbon dioxide.
J Dairy Res. 2000 Nov;67(4):641-6. doi: 10.1017/s0022029900004490.
7
Isolation and properties of Lactococcus lactis subsp. lactis biovar diacetylactis CNRZ 483 mutants producing diacetyl and acetoin from glucose.乳酸乳球菌乳酸亚种双乙酰变种CNRZ 483从葡萄糖产生双乙酰和乙偶姻的突变体的分离及特性
Appl Environ Microbiol. 1997 Jun;63(6):2293-9. doi: 10.1128/aem.63.6.2293-2299.1997.
8
Synthesis of catalase by "Streptococcus faecalis subsp. zymogenes".“粪肠球菌发酵亚种”过氧化氢酶的合成
Arch Microbiol. 1983 Oct;136(1):60-3. doi: 10.1007/BF00415611.
9
Formation of hydrogen peroxide by group N streptococci and its effect on their growth and metabolism.N群链球菌产生过氧化氢及其对其生长和代谢的影响。
Appl Microbiol. 1970 Apr;19(4):608-12. doi: 10.1128/am.19.4.608-612.1970.
10
Altered metabolism in a Streptococcus lactis C2 mutant deficient in lactic dehydrogenase.
J Dairy Sci. 1974 Feb;57(2):181-6. doi: 10.3168/jds.S0022-0302(74)84857-5.
Zotero 插件