CBP/P300在核受体信号传导中的作用。

Role of CBP/P300 in nuclear receptor signalling.

作者信息

Chakravarti D, LaMorte V J, Nelson M C, Nakajima T, Schulman I G, Juguilon H, Montminy M, Evans R M

机构信息

The Gene Expression Laboratory, the Howard Hughes Medical Institute, La Jolla, California 92037, USA.

出版信息

Nature. 1996 Sep 5;383(6595):99-103. doi: 10.1038/383099a0.

DOI:10.1038/383099a0

PMID:8779723

Abstract

The nuclear receptor superfamily includes receptors for steroids, retinoids, thyroid hormone and vitamin D, as well as many related proteins. An important feature of the action of the lipophilic hormones and vitamins is that the maintenance of homeostatic function requires both intrinsic positive and negative regulation. Here we provide in vitro and in vivo evidence that identifies the CREB-binding protein (CBP) and its homologue P300 (refs 6,7) as cofactors mediating nuclear-receptor-activated gene transcription. The role of CBP/P300 in the transcriptional response to cyclic AMP, phorbol esters, serum, the lipophilic hormones and as the target of the E1A oncoprotein suggests they may serve as integrators of extracellular and intracellular signalling pathways.

摘要

核受体超家族包括类固醇、视黄酸、甲状腺激素和维生素D的受体，以及许多相关蛋白。亲脂性激素和维生素作用的一个重要特征是，稳态功能的维持需要内在的正调控和负调控。在此，我们提供了体外和体内证据，确定了CREB结合蛋白（CBP）及其同源物P300（参考文献6,7）作为介导核受体激活基因转录的辅因子。CBP/P300在对环磷酸腺苷、佛波酯、血清、亲脂性激素的转录反应中的作用，以及作为E1A癌蛋白的靶点，表明它们可能作为细胞外和细胞内信号通路的整合者。