Anzai N, Kawabata H, Hishita T, Ueda Y, Yoshida Y
Department of Medicine, Faculty of Medicine, Kyoto University.
Rinsho Ketsueki. 1996 Jul;37(7):536-41.
We examined the endonuclease activity capable of inducing internucleosomal DNA fragmentation in hematopoietic cells. Mg(2+)-dependent nuclease activity was high in hematopoietic progenitor cells and the activity decreased with myeloid or erythroid differentiation. This was the case in MDS as well as in normal hematopoiesis. In contrast, Ca2+/Mg(2+)-dependent nuclease activity varied widely in the samples from MDS and the possibility was indicated that the activity of Glycophorin A+ cells was related to the degree of anemia. We also investigated DNA strand breaks in bone marrow samples from 16 patients with MDS and 10 with other diseases by an in situ end labeling (ISEL) technique. The reactivity in ISEL tended to increase parallel to disease progression of MDS. The high ISEL-positivity was also observed in some samples from patients with MPD and other diseases. Though ISEL is a useful technique for quantification of apoptosis, our results suggested that MDS cells with ISEL positive staining are not necessarily in the process of apoptosis.
我们检测了造血细胞中能够诱导核小体间DNA片段化的核酸内切酶活性。Mg(2+)依赖性核酸酶活性在造血祖细胞中较高，且随着髓系或红系分化而降低。骨髓增生异常综合征（MDS）以及正常造血过程中均是如此。相比之下，MDS样本中Ca2+/Mg(2+)依赖性核酸酶活性差异很大，这表明血型糖蛋白A+细胞的活性与贫血程度有关。我们还通过原位末端标记（ISEL）技术研究了16例MDS患者和10例其他疾病患者骨髓样本中的DNA链断裂情况。ISEL中的反应性倾向于随着MDS疾病进展而平行增加。在一些骨髓增殖性疾病（MPD）患者和其他疾病患者的样本中也观察到高ISEL阳性率。虽然ISEL是定量凋亡的一种有用技术，但我们的结果表明，ISEL阳性染色的MDS细胞不一定处于凋亡过程中。
