EF-Tu [G222D]的转译活性与多肽链延伸循环的经典模式不相符。

Translational activities of EF-Tu [G222D] which cannot be reconciled with the classical scheme of the polypeptide chain elongation cycle.

作者信息

Talens A, Boon K, Kraal B, Bosch L

机构信息

Department of Biochemistry, Leiden Institute of Chemistry, Gorlaeus Laboratories, Leiden University, The Netherlands.

出版信息

Biochem Biophys Res Commun. 1996 Aug 23;225(3):961-7. doi: 10.1006/bbrc.1996.1279.

DOI:10.1006/bbrc.1996.1279

PMID:8780718

Abstract

We have developed a cell-free system of E. coli that enables us to study the in vitro translation of natural mRNA mediated by wild-type or mutant EF-Tu. Various mutant EF-Tu species have been analyzed, one of which, EF-Tu [G222D], appeared to be virtually unable to mediate the translation of natural mRNA. Since this mutant factor is able to participate in translation in vivo by suppressing nonsense and frameshift mutations in cooperation with EF-Tu [A375T], a revision of the generally accepted scheme of the elongation cycle has been proposed (Bosch, L., Vijgenboom, E., & Zeef, L.A.H., 1996, Biochemistry 36).

摘要

我们开发了一种大肠杆菌无细胞系统，该系统使我们能够研究由野生型或突变型EF-Tu介导的天然mRNA的体外翻译。我们分析了多种突变型EF-Tu，其中一种EF-Tu [G222D]似乎几乎无法介导天然mRNA的翻译。由于这种突变因子能够通过与EF-Tu [A375T]协同作用抑制无义突变和移码突变来参与体内翻译，因此有人提出了对普遍接受的延伸循环机制的修订方案（Bosch, L., Vijgenboom, E., & Zeef, L.A.H., 1996, Biochemistry 36）。