Fontanini G, Vignati S, Bigini D, Lucchi M, Mussi A, Basolo F, Angeletti C A, Bevilacqua G
Department of Pathology, University of Pisa, Italy.
Int J Cancer. 1996 Sep 4;67(5):615-9. doi: 10.1002/(SICI)1097-0215(19960904)67:5<615::AID-IJC4>3.0.CO;2-X.
Several studies have documented a relevant prognostic role of microvessel count (MC) in non-small-cell lung carcinomas (NSCLC). However, no evidence has been reported about the involvement of neo-angiogenesis in the development of bronchial cancers. The aim of this study was to analyze microvessel density both in normal and in pathological features of the bronchial tree detected concomitantly with carcinomas. In a group of 34 patients resected for NSCLC, 48 bronchial lesions (hyperplasia, squamous metaplasia, moderate dysplasia and in situ carcinoma) were identified. In addition, 20 samples of normal bronchial epithelium from the same patients were analyzed. A monoclonal antibody was used in order to identify microvessels in the most intense areas of neovascularization from the bronchial specimens. MC was also analyzed in invasive components. An increased number of microvessels was observed from normal to dysplastic epithelium, including in situ carcinoma. Mean MC was significantly lower in normal, hyperplastic and squamous metaplastic epithelium than in dysplastic epithelium and in situ carcinoma. In particular, no differences were observed between normal and hyperplastic/metaplastic components, whereas a statistically significant difference appeared between the latter and dysplastic lesions. Moderate dysplasia and in situ carcinoma showed a number of microvessels in the lamina propria of their mucosa which were not significantly different from the invasive component, whereas hyperplastic/metaplastic lesions presented a much lower number of microvessels than invasive cancer. From these data it appears that normal bronchial epithelium and lesions associated with cancers of the bronchial tree show neovascularization in their stromal component. Hyperplasia and squamous metaplasia, unlike dysplasia and in situ carcinoma, show a low microvessel count, and they cannot represent precursor or incipient changes in the bronchial epithelium before the fully developed in situ stage has also been reached.
多项研究已证明微血管计数（MC）在非小细胞肺癌（NSCLC）中具有重要的预后作用。然而，尚无证据表明新生血管生成与支气管癌的发生有关。本研究的目的是分析与癌同时检测到的支气管树正常和病理特征中的微血管密度。在一组因NSCLC接受手术切除的34例患者中，识别出48个支气管病变（增生、鳞状化生、中度发育异常和原位癌）。此外，还分析了同一患者的20份正常支气管上皮样本。使用单克隆抗体来识别支气管标本中新生血管最密集区域的微血管。还对浸润成分中的MC进行了分析。从正常上皮到发育异常上皮，包括原位癌，观察到微血管数量增加。正常、增生和鳞状化生上皮中的平均MC显著低于发育异常上皮和原位癌。特别是，正常与增生/化生成分之间未观察到差异，而后两者与发育异常病变之间出现了统计学上的显著差异。中度发育异常和原位癌在其黏膜固有层中的微血管数量与浸润成分无显著差异，而增生/化生病变的微血管数量远低于浸润癌。从这些数据看来，正常支气管上皮以及与支气管树癌相关的病变在其基质成分中显示出新生血管生成。增生和鳞状化生与发育异常和原位癌不同，微血管计数较低，并且在完全发展到原位阶段之前，它们不能代表支气管上皮的前驱或初期变化。
