Takeuchi T, Sasaki Y, Ueki T, Kaziwara T, Moriyama N, Kawabe K, Kakizoe T
Department of Urology, Faculty of Medicine, University of Tokyo, Japan.
Int J Cancer. 1996 Sep 4;67(5):709-14. doi: 10.1002/(SICI)1097-0215(19960904)67:5<709::AID-IJC20>3.0.CO;2-Z.
Fas/APO-1 is a cell-surface protein, a member of the TNF-receptor family, and it potentially induces apoptosis. In presence of an apoptosis-inducible anti-human Fas MAb, Fas-negative control PC-3 human prostate-cancer cells did not undergo morphological changes, while PC-3 human Fas transfectants showed apoptotic changes in vitro. However, LNCaP human Fas transfectants, as well as Fas-negative control LNCaP human prostate-cancer cells, were Fas-resistant. The growth of Fas-transfected PC-3 tumor was retarded compared with that of control PC-3 tumor in vivo without stimulation of anti-human Fas MAb. Anti-human Fas MAb administration in vivo caused macroscopic Fas-transfected PC-3 tumors formed in BALB/c nude mice to undergo apoptosis.
Fas/APO-1是一种细胞表面蛋白，属于肿瘤坏死因子受体家族成员，具有潜在诱导细胞凋亡的作用。在存在可诱导细胞凋亡的抗人Fas单克隆抗体的情况下，Fas阴性对照的PC-3人前列腺癌细胞未发生形态学改变，而PC-3人Fas转染细胞在体外呈现出凋亡变化。然而，LNCaP人Fas转染细胞以及Fas阴性对照的LNCaP人前列腺癌细胞对Fas具有抗性。在体内未用抗人Fas单克隆抗体刺激的情况下，与对照PC-3肿瘤相比，Fas转染的PC-3肿瘤生长受到抑制。在体内给予抗人Fas单克隆抗体可使BALB/c裸鼠体内形成的宏观Fas转染PC-3肿瘤发生凋亡。
