T淋巴细胞识别由golli-髓鞘碱性蛋白基因编码的蛋白质的能力与品系相关的差异。

Strain-related differences in the ability of T lymphocytes to recognize proteins encoded by the golli-myelin basic protein gene.

作者信息

Pitchekian-Halabi H, Campagnoni C W, Skinner E, Kampf K, Campagnoni A T, Voskuhl R R

机构信息

Reed Neurological Research Center, UCLA Dept. of Neurology 90024, USA.

出版信息

J Neuroimmunol. 1996 Aug;68(1-2):121-9. doi: 10.1016/0165-5728(96)00084-7.

DOI:10.1016/0165-5728(96)00084-7

PMID:8784268

Abstract

Protein products of the golli-MBP gene complex, expressed in the nervous and lymphoid systems, contain sequences in common with sequences in 'classic' MBP, expressed exclusively in the nervous system. In this report, it was determined whether T cell lines (TCLs) specific for encephalitogenic epitopes of 'classic' MBP were able to recognize sequences within golli-MBP. TCLs derived from SJL mice specific for the immunodominant 83-102 sequence and the subdominant 19-27 sequence of 'classic' MBP recognized golli-MBP J37 and BG21, respectively. In contrast, TCLs derived from PL and B10.PL mice specific for the immunodominant 1-9 sequence of 'classic' MBP did not recognize this sequence within either J37 or BG21. These strain-related differences in the ability of golli-MBP proteins to stimulate 'classic' MBP-specific TCLs are discussed with respect to a possible influence on whether the course of EAE is relapsing (SJL) or monophasic (PL and B10.PL).

摘要

在神经和淋巴系统中表达的golli - MBP基因复合体的蛋白质产物，含有与仅在神经系统中表达的“经典”MBP中的序列相同的序列。在本报告中，确定了对“经典”MBP的致脑炎表位具有特异性的T细胞系（TCLs）是否能够识别golli - MBP中的序列。源自SJL小鼠的针对“经典”MBP的免疫显性83 - 102序列和亚显性19 - 27序列的TCLs，分别识别golli - MBP J37和BG21。相反，源自PL和B10.PL小鼠的针对“经典”MBP的免疫显性1 - 9序列的TCLs，在J37或BG21中均未识别该序列。就对实验性自身免疫性脑脊髓炎（EAE）病程是复发型（SJL）还是单相型（PL和B10.PL）的可能影响，讨论了golli - MBP蛋白刺激“经典”MBP特异性TCLs能力的这些品系相关差异。