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通过醋酸纤维素电泳法测定血迹中磷酸葡萄糖变位酶(PGM1)的表型

Determination of phenotypes of phosphoglucomutase (PGM1) in bloodstains by cellulose acetate electrophoresis.

作者信息

Miścicka D, Dobosz T, Raszeja S

出版信息

Z Rechtsmed. 1977 May 27;79(4):297-300. doi: 10.1007/BF00201172.

DOI:10.1007/BF00201172
PMID:878670
Abstract

A good separation of PGM1 isoenzymes from bloodstains has been obtained with an adapted Sonneborn method of electrophoresis on cellulose acetate. This method requires very small quantities of the examined material, a short time of electrophoresis, and is cheaper and simpler than hitherto described. A correct determination of PGM1 phenotypes from bloodstains was easy up to 24 weeks. Occasionally it was possible to determine phenotypes in bloodstains as old as 47 weeks, and in one case even in bloodstains 126 weeks old. This method may be useful in the forensic serology for determination of PGM1 phenotypes from bloodstains.

摘要

采用改良的索内伯恩醋酸纤维素电泳法,已实现从血迹中良好分离PGM1同工酶。该方法所需检测材料量极少,电泳时间短,且比迄今所描述的方法更便宜、更简便。在长达24周的时间内,从血迹中准确测定PGM1表型很容易。偶尔也有可能在长达47周的血迹中测定表型,在一个案例中甚至在126周龄的血迹中也能测定。该方法在法医血清学中对于从血迹中测定PGM1表型可能有用。

相似文献

1
Determination of phenotypes of phosphoglucomutase (PGM1) in bloodstains by cellulose acetate electrophoresis.通过醋酸纤维素电泳法测定血迹中磷酸葡萄糖变位酶(PGM1)的表型
Z Rechtsmed. 1977 May 27;79(4):297-300. doi: 10.1007/BF00201172.
2
Typing of phosphoglucomutase (PGM) variants in dried bloodstains by the Grunbaum method of cellulose acetate electrophoresis.采用醋酸纤维素电泳的格伦鲍姆方法对干血斑中的磷酸葡萄糖变位酶(PGM)变体进行分型。
J Forensic Sci Soc. 1975 Jan;15(1):69-74. doi: 10.1016/s0015-7368(75)70938-6.
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A comparison of the sensitivity of typing group-specific component (Gc) and the phosphoglucomutase (PGM1) locus in control and casework bloodstains by three isoelectric focusing methods.采用三种等电聚焦方法对对照血痕和案件血痕中血型特异性成分(Gc)和磷酸葡萄糖变位酶(PGM1)位点分型的敏感性比较。
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[Determination of foetal haemoglobin in bloodstains by means of electrophoresis on cellulose acetate (author's transl)].用醋酸纤维素电泳法测定血迹中的胎儿血红蛋白(作者译)
Z Rechtsmed. 1977 Mar 23;79(2):79-80. doi: 10.1007/BF00200232.

本文引用的文献

1
PHOSPHOGLUCOMUTASE POLYMORPHISM IN MAN.人类中的磷酸葡萄糖变位酶多态性
Nature. 1964 Nov 21;204:742-5. doi: 10.1038/204742a0.
2
A thin-layer starch gel method for enzyme typing of bloodstains.一种用于血迹酶分型的薄层淀粉凝胶法。
J Forensic Sci Soc. 1968 Oct;8(2):81-2. doi: 10.1016/s0015-7368(68)70449-7.
3
The determination of phosphoglucomutase (PGM) types in bloodstains.血迹中磷酸葡萄糖变位酶(PGM)类型的测定。
J Forensic Sci Soc. 1967 Jul;7(3):131-3. doi: 10.1016/s0015-7368(67)70393-x.
4
The effect of storage upon the activity of phosphoglucomutase and adenylate kinase enzymes in blood samples and bloodstains.储存对血液样本和血迹中磷酸葡萄糖变位酶及腺苷酸激酶活性的影响。
Med Sci Law. 1970 Oct;10(4):230-4. doi: 10.1177/002580247001000407.
5
[Demonstration of the phenotype of the polymorphous erythrozyte enzymes, acid erythrocyte phosphatase, phosphoglucomutase, adenylate kinaseand adenosine desamylase in 2 steps].[分两步展示多形性红细胞酶、酸性红细胞磷酸酶、磷酸葡萄糖变位酶、腺苷酸激酶和腺苷脱氨酶的表型]
Blut. 1970 Oct;21(4):258-9. doi: 10.1007/BF01632309.
6
[Thin-layer starch gel electrophoresis for determination of phosphoglucomutase types in blood traces].[用于测定血痕中磷酸葡萄糖变位酶类型的薄层淀粉凝胶电泳]
Z Rechtsmed. 1970;67(5):309-12. doi: 10.1007/BF02054016.
7
[Determination of phosphoglucomutase types in blood traces].[血痕中磷酸葡萄糖变位酶类型的测定]
Dtsch Z Gesamte Gerichtl Med. 1969;66(2):31-4.
8
Determination of three enzyme polymorphisms in one electrophoretic step on horizontal polyacrylamide gels.在水平聚丙烯酰胺凝胶上通过一步电泳法测定三种酶的多态性。
Humangenetik. 1971;13(3):250-2. doi: 10.1007/BF00326952.
9
Horizontal polyacrylamide electrophoresis for the determination of serum protein (haptoglobin) and red cell enzyme polymorphisms.用于测定血清蛋白(触珠蛋白)和红细胞酶多态性的水平聚丙烯酰胺电泳
Humangenetik. 1972;14(3):224-31. doi: 10.1007/BF00278041.
10
Comments on the determination of isoenzyme polymorphism (ADA, AK, 6-PGD, PGM) by cellulose acetate electrophoresis.关于用醋酸纤维素电泳法测定同工酶多态性(ADA、AK、6 - PGD、PGM)的评论
Humangenetik. 1972;17(1):49-55. doi: 10.1007/BF01789599.