Carrier N, Connat J L
Anatomie et Physiologie Comparées, Université de Genève, Switzerland.
Neurobiol Aging. 1996 Jan-Feb;17(1):53-60. doi: 10.1016/0197-4580(95)02027-6.
Calcitonin gene-related peptide (CGRP) innervation and binding sites were studied on hepatic portal veins of male and female rats from 19 days to 22 months of age. CGRP containing nerve fibers were present both in adventitial and medial nerve plexuses, closely apposing to or penetrating into the muscular layers. The density of CGRP innervation was estimated on whole mount preparations and compared during aging. In females, aging did not affect the number of fibers per unit length, although the vessel circumference decreased after 6 months of age. In males, the vessel circumference remained constant during aging, while the density of innervation significantly decreased. Whatever the age or sex of the animals was, no CGRP binding sites were found on portal veins sections by autoradiography. CGRP had no effect on spontaneous contractions of perfused portal veins. The difference observed in the evolution of CGRP innervation density between males and females suggests that CGRP innervation in the rat portal vein may be influenced by gonadal steroids during aging.
对19日龄至22月龄的雄性和雌性大鼠肝门静脉的降钙素基因相关肽（CGRP）神经支配和结合位点进行了研究。含CGRP的神经纤维存在于外膜和中膜神经丛中，与肌层紧密相邻或穿透肌层。在整装标本上估计CGRP神经支配密度，并在衰老过程中进行比较。在雌性大鼠中，衰老并不影响单位长度的纤维数量，尽管6月龄后血管周长减小。在雄性大鼠中，衰老过程中血管周长保持不变，而神经支配密度显著降低。无论动物的年龄或性别如何，通过放射自显影在门静脉切片上均未发现CGRP结合位点。CGRP对灌注门静脉的自发收缩没有影响。雄性和雌性大鼠在CGRP神经支配密度变化方面的差异表明，衰老过程中大鼠门静脉的CGRP神经支配可能受性腺类固醇的影响。
