Thiévent A, Connat J L
Université de Genève, Anatomie et Physiologie Comparées, Switzerland.
J Auton Nerv Syst. 1993 Aug-Sep;44(2-3):233-41. doi: 10.1016/0165-1838(93)90036-t.
Indirect immunohistochemistry performed on whole mounts of arch and thoracic part of the rat aorta at six developmental stages (from embryonic day 17 to 6 months, in males and females) revealed that calcitonin gene-related peptide (CGRP) innervation is highest in the arch. The highest density of innervation is found at the three first postnatal ages investigated (day 1, day 3 and 5 weeks; 2.6 +/- 0.6 intercepts/mm in the arch at 1 day); however, all values are low compared to other arteries. The innervation grows from a few short isolated fibres in the embryo to a more complex meshwork in older animals. No striking differences were noticed between males and females. Autoradiographic studies were performed on serial sections at several levels of the aorta but did not reveal binding sites for CGRP in the vascular wall. This might be due to the technique which does not allow visualization of low density of binding sites, or to binding sites of weak affinity. We discuss the possible importance of CGRP in rat aortic smooth muscle development.
对大鼠主动脉弓和胸部的整个标本在六个发育阶段（从胚胎第17天到6个月，包括雄性和雌性）进行间接免疫组织化学分析，结果显示降钙素基因相关肽（CGRP）神经支配在主动脉弓中最为丰富。在所研究的出生后的前三个年龄段（第1天、第3周和第5周；出生1天时主动脉弓的神经支配密度为2.6±0.6个交点/毫米）发现神经支配密度最高；然而，与其他动脉相比，所有这些值都较低。神经支配从胚胎期的几根短的孤立纤维发展到老年动物中更复杂的网络结构。未观察到雄性和雌性之间有明显差异。对主动脉几个水平的连续切片进行放射自显影研究，但未在血管壁中发现CGRP的结合位点。这可能是由于该技术无法显示低密度的结合位点，或者是由于亲和力较弱的结合位点。我们讨论了CGRP在大鼠主动脉平滑肌发育中的可能重要性。
