普通嗜热放线菌产α-淀粉酶的研究。

Studies on the formation of alpha-amylase by Thermomonospora vulgaris.

作者信息

Allam A M, Hussein A M, Ragab A M

出版信息

Zentralbl Bakteriol Parasitenkd Infektionskr Hyg. 1977;132(2):143-7. doi: 10.1016/s0044-4057(77)80056-7.

Abstract

Conditions affecting the formation of alpha-amylase by static cultures of the thermophilic actinomycete Thermomonospora vulgaris were studied. The organism failed to grow under submerged culture conditions or when the culture medium was devoid of CaCO3-alpha-Amylase was produced during the logarithmic phase of growth and maximum yield was obtained after 3 to 9 days of incubation. Growth and amylase formation took place only in a range from 45 degrees to 55 degrees C; optimum temperature was 55 degrees C. Of the tested carbon sources only starch induced enzyme formation. Maximum enzyme yield was obtained when starch concentration of the medium was 2% and when ammonium citrate served as a nitrogen source. Crushed clay pots could substitute for CaCO3 of the medium, but growth and amylase yield were less.

摘要

研究了嗜热放线菌普通嗜热单孢菌静态培养条件下影响α-淀粉酶形成的因素。该微生物在深层培养条件下或培养基中不含碳酸钙时无法生长。α-淀粉酶在生长的对数期产生，培养3至9天后获得最大产量。生长和淀粉酶形成仅发生在45℃至55℃的范围内；最适温度为55℃。在所测试的碳源中，只有淀粉能诱导酶的形成。当培养基中淀粉浓度为2%且柠檬酸铵作为氮源时，可获得最大酶产量。碎陶罐可替代培养基中的碳酸钙，但生长和淀粉酶产量较低。