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多食性根腐病菌的生理学研究。十二、培养条件对淀粉酶合成的影响。

Physiological studies on Phymatotrichum omnivorum. XII Influence of cultural conditions on amylase synthesis.

作者信息

Gunasekaran M

出版信息

Microbios. 1981;29(115):37-43.

PMID:6166833
Abstract

The production of extracellular amylase by P. omnivorum has been studied under different cultural conditions. Maximum mycelial growth and amylase synthesis were found in basal synthetic medium containing 2% glycogen as the carbon source. Significant amounts of amylase and growth were obtained in medium containing other carbon compounds with alpha-1, 4-linkages. Optimum temperatures for mycelial growth and amylase synthesis were found at 28 degree and 30 degree C, respectively. Of all the nitrogenous compounds tested, ammonium nitrate supported the maximum mycelial growth and amylase production. Increasing the concentration of starch in the basal medium increased the growth as well as the enzyme synthesis.

摘要

已在不同培养条件下研究了杂食假丝酵母产生胞外淀粉酶的情况。在以2%糖原作为碳源的基础合成培养基中,发现菌丝体生长和淀粉酶合成达到最大值。在含有其他具有α-1,4-糖苷键的碳化合物的培养基中,可获得大量的淀粉酶并实现生长。发现菌丝体生长和淀粉酶合成的最适温度分别为28℃和30℃。在所有测试的含氮化合物中,硝酸铵支持最大的菌丝体生长和淀粉酶产生。增加基础培养基中淀粉的浓度可促进生长以及酶的合成。

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