Soni S K, Sandhu I K, Bath K S, Banerjee U C, Patnaik P R
Department of Microbiology, Panjab University, Chandigarh, India.
Folia Microbiol (Praha). 1996;41(3):243-8. doi: 10.1007/BF02814624.
A strain of starch-assimilating yeast, Saccharomycopsis capsularis, isolated from Indian cereal-based fermented foods, produced significant levels of extracellular alpha-amylase and glucoamylase. The enzymes reached their peak activities during the stationary phase at the end of the 5th and 4th day of cultivation, respectively. The amylase yields were maximized by a proper choice of carbon and nitrogen sources, starting pH of the culture medium and growth temperature. High activities of the enzymes were obtained through inexpensive agricultural commodities, such as wheat bran and corn meal as carbon sources, and defatted soybean meal and peanut meal as nitrogen sources. A temperature of 28-32 degrees C and an initial pH of 4.5-5.0 were optimum. The crude amylase mixture could liquefy and saccharify a 1% starch solution completely in 24 h at 50 degrees C.
从印度谷类发酵食品中分离出的一株能同化淀粉的酵母——荚膜内孢霉，可产生大量胞外α-淀粉酶和糖化酶。这些酶分别在培养第5天和第4天结束时的稳定期达到最高活性。通过适当选择碳源和氮源、培养基起始pH值和生长温度，可使淀粉酶产量最大化。以麦麸和玉米粉等廉价农产品作为碳源，脱脂豆粕和花生粕作为氮源，可获得高活性的酶。28 - 32摄氏度的温度和4.5 - 5.0的初始pH值为最佳条件。粗淀粉酶混合物在50摄氏度下24小时内可将1%的淀粉溶液完全液化和糖化。
