Birkett A M, Jones G P, Muir J G
School of Nutrition and Public Health, Deakin University, Geelong, Victoria, Australia.
J Chromatogr B Biomed Appl. 1995 Dec 15;674(2):187-91. doi: 10.1016/0378-4347(95)00324-x.
Previous reports which present methods of analysis of phenol and p-cresol by HPLC are usually designed for the detection of these compounds in urine, can be complicated by the use of uncommon equipment or additional techniques such as steam distillation or derivatisation, or concentrate on the detection of phenol rather than p-cresol. In this paper we report a simple method suitable for the analysis of phenol and p-cresol in both urine and feces, based upon extraction into ether following acid hydrolysis and UV detection.
以往报道的采用高效液相色谱法分析苯酚和对甲酚的方法通常是为检测尿液中的这些化合物而设计的，可能会因使用不常见的设备或额外技术（如蒸汽蒸馏或衍生化）而变得复杂，或者侧重于苯酚而非对甲酚的检测。在本文中，我们报告了一种简单的方法，该方法基于酸水解后用乙醚萃取及紫外检测，适用于分析尿液和粪便中的苯酚和对甲酚。
