Niwa T
Department of Clinical Laboratory, Nagoya University Branch Hospital, Japan.
Clin Chem. 1993 Jan;39(1):108-11.
We developed a simple and sensitive high-performance liquid chromatographic (HPLC) method that uses fluorescence as a detector for quantifying serum phenol and p-cresol in uremic patients on hemodialysis. Identification of phenol and p-cresol was confirmed by liquid chromatography/mass spectrometry. Because the HPLC method requires only simple extraction by ethyl acetate and does not require further steps such as derivatization, it is simple and rapid compared with gas chromatography or gas chromatography/mass spectrometry. Concentrations of phenol and p-cresol in uremic serum were significantly (p < 0.01) higher than those in normal serum. Reduction rates of phenol and p-cresol by hemodialysis were lower than those of urea and creatinine, suggesting a protein-binding property of phenol and p-cresol. This method will be useful for monitoring serum phenols in dialyzed patients as an index of hemodialysis adequacy.
我们开发了一种简单且灵敏的高效液相色谱（HPLC）方法，该方法使用荧光作为检测器来定量血液透析的尿毒症患者血清中的苯酚和对甲酚。通过液相色谱/质谱法确认了苯酚和对甲酚的鉴定。由于HPLC方法仅需要用乙酸乙酯进行简单提取，不需要衍生化等进一步步骤，与气相色谱或气相色谱/质谱法相比，它简单且快速。尿毒症血清中苯酚和对甲酚的浓度显著高于正常血清（p < 0.01）。血液透析对苯酚和对甲酚的清除率低于尿素和肌酐，表明苯酚和对甲酚具有蛋白结合特性。该方法将有助于监测透析患者的血清酚类，作为血液透析充分性的指标。
