Madhu C, Gregus Z, Klaassen C D
Department of Pharmacology, Toxicology and Therapeutics, University of Kansas Medical Center, Kansas City 66160-7417, USA.
J Chromatogr B Biomed Appl. 1995 Dec 15;674(2):193-6. doi: 10.1016/0378-4347(95)00306-1.
A simple HPLC method has been described to quantify diquat in biological fluids and tissues. This method permits separation and quantification of diquat from blood, bile, urine, liver and kidney. It does not require special pretreatment of the samples prior to analysis, nor a specially prepared analytical column. Various concentrations of diquat were added (10-300 nmol/ml or g) to fluids or tissues. Analysis of blank samples revealed no substances that interfere with diquat elution. Excellent recovery (95-105%) was obtained. Diquat (120 mumol/kg, i.v.) was injected to rats and quantified in bile, blood and liver. Concentration of diquat was higher in blood and bile than liver. Therefore, this method is applicable for quantification of diquat in toxicological samples, and may be used to determine structurally similar compounds such as paraquat.
已描述了一种简单的高效液相色谱法用于定量生物体液和组织中的敌草快。该方法可实现从血液、胆汁、尿液、肝脏和肾脏中分离并定量敌草快。分析前无需对样品进行特殊预处理，也不需要特别制备的分析柱。向体液或组织中加入不同浓度的敌草快（10 - 300 nmol/ml或g）。空白样品分析显示没有干扰敌草快洗脱的物质。回收率极佳（95 - 105%）。给大鼠静脉注射敌草快（120 μmol/kg），并在胆汁、血液和肝脏中进行定量。敌草快在血液和胆汁中的浓度高于肝脏。因此，该方法适用于毒理学样品中敌草快的定量，也可用于测定结构相似的化合物，如百草枯。
