Ryd W, Hagmar B
Acta Pathol Microbiol Scand A. 1977 May;85(3):405-12. doi: 10.1111/j.1699-0463.1977.tb00442.x.
We have studied how the presence of cell aggregates affects the pattern of intravenously induced "experimental metastases" from two syngeneic murine tumors. Aggregates were produced mechanically by centrifugation, or chemically by a lectin (wheat-germ agglutinin). Compared to well-dissociated suspensions, aggregated suspensions tended to give a greater total metastasis volume in the lungs of recipient mice. Disaggregated suspensions, on the other hand, gave rise to more extrapulmonary metastases. Presumably, aggregates are preferentially retained in lung vessels, while single cells are let through to other sites. Nor are aggregates superior to single cells in producing tumor growth when the total metastasis yield is considered.
我们研究了细胞聚集体的存在如何影响两种同基因小鼠肿瘤静脉诱导的“实验性转移”模式。聚集体通过离心机械产生，或通过凝集素（麦胚凝集素）化学产生。与充分解离的悬浮液相比，聚集的悬浮液在受体小鼠肺部往往产生更大的总转移体积。另一方面，解离的悬浮液会导致更多的肺外转移。据推测，聚集体优先滞留在肺血管中，而单个细胞则会流向其他部位。当考虑总转移产量时，聚集体在产生肿瘤生长方面也并不优于单个细胞。
