IRK1内向整流钾通道疏水结构域中带负电荷残基对钾离子选择性通透的作用。
Contributions of a negatively charged residue in the hydrophobic domain of the IRK1 inwardly rectifying K+ channel to K(+)-selective permeation.
作者信息
Reuveny E, Jan Y N, Jan L Y
机构信息
Department of Physiology, University of California, San Francisco 94143-0724, USA.
出版信息
Biophys J. 1996 Feb;70(2):754-61. doi: 10.1016/S0006-3495(96)79615-7.
Abstract
Inwardly rectifying K+ channels are highly selective for K+ ions and show strong interaction with ions in the pore. Both features are important for the physiological functions of these channels and pose intriguing mechanistic questions of ion permeation. The aspartate residue in the second putative transmembrane segment of the IRK1 inwardly rectifying K+ channel, previously implicated in inward rectification gating due to cytoplasmic Mg2+ and polyamine block, is found in this study to be crucial for the channel's ability to distinguish between K+ and Rb+ ions. Mutation of this residue also perturbs the interaction between the channel pore and the Sr2+ blocking ion. Our studies suggest that this aspartate residue contributes to a selectivity filter near the cytoplasmic end of the pore.
摘要
内向整流钾通道对钾离子具有高度选择性，并在孔道中与离子表现出强烈相互作用。这两个特性对于这些通道的生理功能都很重要，并且提出了关于离子渗透的有趣机制问题。在本研究中发现，IRK1内向整流钾通道第二个假定跨膜片段中的天冬氨酸残基，先前被认为因胞质镁离子和多胺阻断而与内向整流门控有关，对于通道区分钾离子和铷离子的能力至关重要。该残基的突变也扰乱了通道孔道与锶离子阻断剂之间的相互作用。我们的研究表明，这个天冬氨酸残基有助于在孔道胞质端附近形成一个选择性过滤器。
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本文引用的文献
1
POTENTIAL, IMPEDANCE, AND RECTIFICATION IN MEMBRANES.膜的电位、阻抗和整流。
J Gen Physiol. 1943 Sep 20;27(1):37-60. doi: 10.1085/jgp.27.1.37.
2
The effect of sodium ions on the electrical activity of giant axon of the squid.钠离子对鱿鱼巨大轴突电活动的影响。
J Physiol. 1949 Mar 1;108(1):37-77. doi: 10.1113/jphysiol.1949.sp004310.
3
Cs(+) causes a voltage-dependent block of inward K currents in resting skeletal muscle fibres.铯离子（Cs(+)）在静息骨骼肌纤维中引起内向钾电流的电压依赖性阻断。
Nature. 1977 May 12;267(5607):169-70. doi: 10.1038/267169a0.
4
The influence of potassium and chloride ions on the membrane potential of single muscle fibres.钾离子和氯离子对单根肌纤维膜电位的影响。
J Physiol. 1959 Oct;148(1):127-60. doi: 10.1113/jphysiol.1959.sp006278.
5
Fast and slow blockades of the inward-rectifier K+ channel by external divalent cations in guinea-pig cardiac myocytes.豚鼠心肌细胞中外源性二价阳离子对内向整流钾通道的快速和慢速阻断
Pflugers Arch. 1993 Feb;422(5):427-35. doi: 10.1007/BF00375067.
6
The internal quaternary ammonium receptor site of Shaker potassium channels.摇椅式钾通道的内部季铵受体位点。
Neuron. 1993 Mar;10(3):533-41. doi: 10.1016/0896-6273(93)90340-w.
7
The S4-S5 loop contributes to the ion-selective pore of potassium channels.S4-S5环对钾通道的离子选择性孔有贡献。
Neuron. 1993 Oct;11(4):739-49. doi: 10.1016/0896-6273(93)90083-4.
8
Segmental exchanges define 4-aminopyridine binding and the inner mouth of K+ pores.片段交换定义了4-氨基吡啶的结合以及钾离子通道的内口。
Neuron. 1993 Sep;11(3):503-12. doi: 10.1016/0896-6273(93)90154-j.
9
Primary structure and functional expression of a rat G-protein-coupled muscarinic potassium channel.大鼠G蛋白偶联毒蕈碱钾通道的一级结构与功能表达
Nature. 1993 Aug 26;364(6440):802-6. doi: 10.1038/364802a0.
10
Conduction properties of the cloned Shaker K+ channel.克隆的Shaker钾离子通道的传导特性。
Biophys J. 1993 Nov;65(5):2089-96. doi: 10.1016/S0006-3495(93)81244-X.
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