性腺激素对灌流大鼠松果体褪黑素释放影响的昼夜差异。昼夜节律控制的证据。

Day-night differences in the effects of gonadal hormones on melatonin release from perifused rat pineals. Evidence of a circadian control.

作者信息

Martin M S, Bogdan A, Touitou Y

机构信息

Department of Biochemistry, Faculté de Médecine Pitié-Salpêtrière, Paris, France.

出版信息

Steroids. 1996 Jan;61(1):27-32. doi: 10.1016/0039-128x(95)00172-m.

DOI:10.1016/0039-128x(95)00172-m

PMID:8789733

Abstract

The effects of testosterone (10(-3) M, 10(-6) M, and 10(-9) M) and 17 beta-estradiol (10(-3) M and 10(-9) M) were looked for upon isoproterenol-stimulated melatonin release by perifused pineal glands removed from male rats and female rats in diestrus. Two different times of a 12/12 hour light/dark cycle, i.e., 7 and 19 hours after light onset, were documented in order to look for the existence of a circadian stage-dependence of the hormone effects. Both testosterone (45-60%) and estradiol (60-80%) markedly increased melatonin release by glands removed during the dark span and not during the light span. These results show a direct effect of gonadal hormones on pineal melatonin release and strongly suggest a time-related effect of these hormones on pineal function.

摘要

研究了睾酮（10⁻³ M、10⁻⁶ M和10⁻⁹ M）以及17β-雌二醇（10⁻³ M和10⁻⁹ M）对从处于动情间期的雄性和雌性大鼠摘除的经灌流松果体中异丙肾上腺素刺激的褪黑素释放的影响。记录了12/12小时光照/黑暗周期的两个不同时间点，即光照开始后7小时和19小时，以探究激素效应是否存在昼夜节律阶段依赖性。睾酮（45%-60%）和雌二醇（60%-80%）均显著增加了在黑暗时段而非光照时段摘除的松果体的褪黑素释放。这些结果表明性腺激素对松果体褪黑素释放有直接作用，并强烈提示这些激素对松果体功能有时间相关效应。