Tuovinen K, Kaliste-Korhonen E, Raushel F M, Hänninen O
Department of Physiology, University of Kuopio, Finland.
Fundam Appl Toxicol. 1996 Jun;31(2):210-7. doi: 10.1006/faat.1996.0093.
The protective effect of phosphotriesterase (PTE) on cholinesterase (ChE) and carboxylesterase (CaE) activities was studied in mice. The PTE pretreatment (120 U/g body wt, 9.6 micrograms/g body wt) given i.v. 10 min before diisopropyl fluorophosphate, sarin, or soman variably prevented ChE inhibition in erythrocytes and plasma and CaE in plasma. PTE also protected the brain and lung ChEs against inactivation by organophosphates (OPs). The recovery of the enzymes was dependent on the OP used. Postexposure therapy with PTE, given 1.5 hr after paraoxon, also prevented ChE inhibition in erythrocytes, brain, and lung 24 hr after exposure. The distribution studies with [125I]PTE showed that PTE does not markedly gain access into the central nervous system.
在小鼠中研究了磷酸三酯酶（PTE）对胆碱酯酶（ChE）和羧酸酯酶（CaE）活性的保护作用。在静脉注射二异丙基氟磷酸酯、沙林或梭曼前10分钟给予PTE预处理（120 U/g体重，9.6微克/g体重），可不同程度地预防红细胞和血浆中ChE的抑制以及血浆中CaE的抑制。PTE还可保护脑和肺中的ChE免受有机磷酸酯（OPs）的灭活作用。酶的恢复情况取决于所使用的OP。在对氧磷暴露1.5小时后给予PTE进行暴露后治疗，也可预防暴露后24小时红细胞、脑和肺中ChE的抑制。用[125I]PTE进行的分布研究表明，PTE不会显著进入中枢神经系统。
