Kvβ1亚基与震颤相关钾通道α亚基特异性结合。

Kv beta 1 subunit binding specific for shaker-related potassium channel alpha subunits.

作者信息

Sewing S, Roeper J, Pongs O

机构信息

Zentrum für Molekulare Neurobiologie Institut für Neurale Signalverarbeitung, Hamburg Federal Republic of Germany.

出版信息

Neuron. 1996 Feb;16(2):455-63. doi: 10.1016/s0896-6273(00)80063-x.

DOI:10.1016/s0896-6273(00)80063-x

PMID:8789960

Abstract

Voltage-activated potassium (Kv) channels from mammalian brain are hetero-oligomers containing alpha and beta subunits. Coexpression of Kv1 alpha and Kv beta 1 subunits confers rapid A-type inactivation on noninactivating potassium channels (delayed rectifiers) in expression systems in vitro. We have delineated a Kv1.5 aminoterminal region of up to 90 amino acids (residues 112-201) that is sufficient for interactions of Kv1.5 alpha and Kv beta 1 subunits. Within this region of the Kv1.5 amino terminus (residues 193-201), a Kv beta 1 interaction site necessary for Kv beta 1-mediated rapid inactivation of Kv1.5 currents was detected. This interaction site motif (FYE/QLGE/DEAM/L) is found exclusively in the Shaker-related subfamily (Kv1). The results show that hetero-oligomerization between alpha and Kv beta 1 subunits is restricted to Shaker-related potassium channel alpha subunits.

摘要

来自哺乳动物大脑的电压激活钾（Kv）通道是包含α和β亚基的异源寡聚体。在体外表达系统中，Kv1α和Kvβ1亚基的共表达赋予非失活钾通道（延迟整流器）快速A型失活特性。我们已经确定了Kv1.5氨基末端长达90个氨基酸（第112 - 201位残基）的区域，该区域足以实现Kv1.5α和Kvβ1亚基的相互作用。在Kv1.5氨基末端的这个区域内（第193 - 201位残基），检测到一个Kvβ1相互作用位点，它是Kvβ1介导的Kv1.5电流快速失活所必需的。这种相互作用位点基序（FYE/QLGE/DEAM/L）仅在与Shaker相关的亚家族（Kv1）中发现。结果表明，α亚基和Kvβ1亚基之间的异源寡聚化仅限于与Shaker相关的钾通道α亚基。