Snowden K C, Buchhholz W G, Hall T C
Institute of Developmental and Molecular Biology, Texas A&M University, College Station 77843-3155, USA.
Plant Mol Biol. 1996 Jun;31(3):689-92. doi: 10.1007/BF00042241.
A series of promoter-GUS fusion constructs containing a portion of the rice triosephosphate isomerase (tpi) promoter, the first tpi intron, and the gene encoding bacterial beta-glucuronidase (GUS) were made. These constructs were electroporated into rice protoplasts and transient expression was monitored. Inclusion of the first intron from the rice tpi gene enhanced expression of the GUS gene from the tpi promoter when it was placed 5' of the GUS gene. When the tpi intron was placed in the 3'-untranslated region no enhancement of GUS gene expression was observed, indicating the importance of position in intron-mediated enhancement of gene expression.
构建了一系列启动子-GUS融合构建体，其中包含水稻磷酸丙糖异构酶（tpi）启动子的一部分、第一个tpi内含子以及编码细菌β-葡萄糖醛酸酶（GUS）的基因。将这些构建体电穿孔导入水稻原生质体并监测瞬时表达。当水稻tpi基因的第一个内含子置于GUS基因的5'端时，它增强了tpi启动子驱动的GUS基因的表达。当tpi内含子置于3'-非翻译区时，未观察到GUS基因表达增强，这表明内含子介导的基因表达增强中位置的重要性。
